Abstract
Inad libitum-fed diabetic rats, sucrase specific activities in jejunum and ileum were significantly increased two- to threefold compared to controls, a response unaltered by pair-feeding. Gradients of sucrase activities along the ileal villus-to-crypt axis were readily measured in crypt regions in diabetic, but not in nondiabetic, rats. Changes in sucrase activities were commensurate with increases in sucrase immunoreactivity and not a result of altered functional activity. Insulin treatment reversed these effects, although insulin-deficiency, studied in food-deprived, nondiabetic rats, did not affect sucrase expression. We conclude that chronic diabetes significantly stimulates sucrase expression along the proximal-to-distal and villus-to-crypt axes of rat small intestine. In ileum, these changes suggest marked alterations in phenotypic development of enterocytes along the villus-to-crypt axis. Alterations in sucrase expression do not appear to correlate with insulin states and are not a consequence of altered functional activity.
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Vinnik IE, Kern F, Sussman KE: The effect of diabetes mellitus and insulin on glucose absorption by the small intestine in man. J Lab Clin Med 66:131–136, 1965
Mooradian AD, Morley JE, Levine AS, Prigge WF, Gebhard RL: Abnormal intestinal permeability to sugars in diabetes mellitus. Diabetologia 29:221–224, 1986
Caspary WF: Effect of insulin and experimental diabetes mellitus on the digestive-absorptive function of the small intestine. Digestion 9:248–263, 1973
Miller DL, Hanson W, Schedl HP, Osborne JW: Proliferation rate and transit time of mucosal cells in small intestine of the diabetic rat. Gastroenterology 73:1326–1332, 1977
Lal D, Schedl HP: Intestinal adaptation in diabetes: Amino acid absorption. Am J Physiol 227:827–831, 1974
Olsen WA, Korsmo H: The intestinal brush border membrane in diabetes. J Clin Invest 60:181–188, 1977
Westergaard H: Insulin modulates rat intestinal glucose transport: Effect of hypoinsulinemia and hyperinsulinemia. Am J Physiol 256:G911-G918, 1989
Chang EB, Bergenstahl RM, Field M: Diarrhea in streptozocin treated rats. J Clin Invest 75:1666–1670, 1985
Tsuboi KT, Kwong LK, Burrill PH, Sunshine P: Sugar hydrolases and their arrangement on the rat intestinal microvillus membrane. J Membr Biol 50:101–122, 1979
Triadou N, Bataille J, Schmitz J: Longitudinal study of the human intestinal brush border membrane proteins. Gastroenterology 85:1326–1332, 1983
Weiser MM: Intestinal epithelial cell surface membrane glycoprotein synthesis. J Biol Chem 248:2536–2541, 1973
Karasov WH, Diamond JM: Adaptive regulation of sugar and amino acid transport by vertebrate intestine. Am J Physiol 245:G443-G462, 1983
Messer M, Dahlquist A: A one step ultramicro method for the assay of intestinal disaccharidases. Anal Biochem 14:376–392, 1966
Garen A, Levinthal C: A fine structure genetic and chemical study of the enzyme alkaline phosphatase ofE. coli. I. Purification and characterization of alkaline phosphatase. Biochim, Biophys Acta 38:470–483, 1960
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951
Brasitus TA, Kerestzes RS: Glycoprotein metabolism in rat colonic epithelial cell populations with different proliferative activities. Differentiation 24:239–244, 1983
Burton K: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–322, 1956
Bonifacio JS: Analysis of proteins.In Current Protocols in Molecular Biology. FM Ausubel, R Brent, R Kingston, et al. (eds). New York, John Wiley & Sons, 1991, pp 10.0.1–10.18.9
Fedorak RN, Gershon MD, Field M: Induction of intestinal glucose carriers in streptozocin-treated chronically diabetic rats. Gastroenterology 96:37–44, 1989
Fedorak RN, Chang EB, Madara JL, Field M: Intestinal adaptation to diabetes: Altered Na-dependent nutrient absorption in streptococin-treated chronically diabetic rats. J Clin Invest 79:1571–1578, 1987
Madara JL, Wolf JL, Trier JS: Structural features of the rat small intestinal microvillus membrane in acute experimental diabetes. Dig Dis Sci 27:801–806, 1982
Caspary WF, Rhein AM, Creutzfeldt W: Increase of intestinal brush border hydrolases in mucosa of streptozotocindiabetic rats. Diabetologia 8:412–414, 1972
Henning SJ: Functional development of the gastrointestinal tract.In Physiology of the Gastrointestinal Tract. LR Johnson (ed). New York, Raven Press, 1987, pp 285–300
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This study was supported by grants from the National Institutes of Health, DK38510 and DK40922 and Digestive Disease Core Center of the University of Chicago (DK42086)
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Hoffman, L.R., Yen, S. & Chang, E.B. Regional alterations in intestinal sucrase expression in streptozocin-treated chronically diabetic rats. Digest Dis Sci 37, 1078–1083 (1992). https://doi.org/10.1007/BF01300290
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DOI: https://doi.org/10.1007/BF01300290