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A canine model for the study of gastric secretion and emptying after a meal

Digestive Diseases and Sciences volume 28, pages 633–640 (1983)Cite this article

Abstract

Our aim was to develop a chronic canine model that would serve for the simultaneous measurements of postprandial gastric secretion and emptying by a double-marker dilution technique without artificially interfering with intragastric pH. A constant duodenal perfusion of a nonabsorbable marker allowed determination of luminal flow and total recovery of a second marker ingested with a meal. By calculating the amount of meal marker remaining in the stomach and its dilution (by repeat gastric sampling), we could determine the volume of gastric contents. Acid concentration in gastric samples was measured by titrationex vivo, and gastric acid content was calculated by multiplying the volume of gastric contents by titratable acidity. In this model, net acid gain at each sampling interval indicates acid output. Acid and volume losses are equivalent to duodenal acid load ad emptying rate, respectively. The method has been validated by virtually abolishing the endogenous secretion of acid (by intravenous infusion of cimetidine) and the calculation of known volumes and concentrations of acid exogenously instilled in the animal's stomach. Our results indicate that this is an accurate and practical method for the physiologic measurement of postprandial gastric function in dogs. Further, these studies substantiate the validity of the assumptions upon which the human double-marker method was based.

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Author information

Author notes

  1. Hussein Kholeif MD

    Present address: Faculty of Medicine, Ain Shams University, Cairo, Egypt

  2. Jorge Larach MD

    Present address: Departmento de Cirugia, Hospital Clinico de la U. de Chile, “J.J. Aguirre,”, Santos Dumon, 999, Santiago, Chile

  3. Juan-R. Malagelada MD

    Present address: Gastroenterology Unit, Mayo Clinic, 55905, Rochester, Minnesota

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    Authors
    1. Hussein Kholeif MD
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    2. Jorge Larach MD
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    3. George M. Thomforde
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    4. Roger R. Dozois MD
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    5. Juan-R. Malagelada MD
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    Additional information

    Supported in part by grant AM-26428 from the National Institutes of Health. Dr. Malagelada was the recipient of RCDA AM-00330 from the National Institutes of Health.

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    Kholeif, H., Larach, J., Thomforde, G.M. et al. A canine model for the study of gastric secretion and emptying after a meal. Digest Dis Sci 28, 633–640 (1983). https://doi.org/10.1007/BF01299924

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    • DOI: https://doi.org/10.1007/BF01299924

    Keywords

    • Cimetidine
    • Titratable Acidity
    • Gastric Secretion
    • Gastric Content
    • Canine Model