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Differences in antigen expression between neoplastic and nonneoplastic gallbladder epithelium

An immunohistochemical study

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Abstract

Immunoreactivity for a panel of 15 monoclonal antibodies (MAbs), which are known to react with different gastrointestinal tumor antigens, was assessed in formalin-fixed paraffinembedded sections that were prepared from cholecystectomy specimens obtained from Mexican patients. Each case was classified histologically into one of the following groups: (1) invasive adenocarcinoma (N=21), (2) high-grade dysplasia (carcinomain situ) (N=2), (3) low-grade dysplasia (N=4), hyperplasia (4) (N=15), and (5) chronic cholecystitis (N=10). Significant differences (P<0.05) were identified among the five histopathologic groups in the proportion of epithelial cells demonstrating immunoreactivity with MAbs to Lewisb; Lewisa; sialylated Lewisa; sialylated Lewisa and Lewisa; Y antigen; H antigen; X antigen; X-like antigen; 200-kDa protein of CEA; 180-, 160-, 50-, 40-kDa proteins of CEA; 30- to 37-kDa protein; and an undefined antigen identified by MAb 99–57, with invasive carcinoma more frequently being positive as compared to nonneoplastic (hyperplasia, chronic cholecystitis) epithelium. Significant differences were also observed among the five histopathologic groups (P<-0.0005) in the proportion of epithelial cells demonstrating immunoreactivity with MAbs to Y antigen and the 20- to 50-kDa glycoprotein. However, with these two antibodies immunoreactivity was more frequently found in nonneoplastic epithelium rather than in invasive carcinomas. No significant differences in immunoreactivity were detected among the different histologic groups with MAb to blood group B antigen, types 1 and 2. This study demonstrates that cellular antigens are both developed and lost during the process of neoplastic transformation in the gallbladder. Future studies including a greater number of cases with varying degrees of gallbladder intraepithelial neoplasia are necessary in order to be able to draw meaningful conclusions regarding the antigenic expression of epithelium at different stages of tumorigenesis.

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Authors and Affiliations

  1. Wistar Institute of Anatomy and Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania

    Dimitrios Iliopoulos MD, Barbara Atkinson MD, Scott H. Saul MD, Meenhard Herlyn DVM, Hector A. Rodriguez-Martinez MD, Suzanne L. West MPH, Greg Maislin BS, MA, Roger D. Soloway MD &  Brian L. Strom MD, MPH

  2. Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania

    Dimitrios Iliopoulos MD, Barbara Atkinson MD, Scott H. Saul MD, Meenhard Herlyn DVM, Hector A. Rodriguez-Martinez MD, Suzanne L. West MPH, Greg Maislin BS, MA, Roger D. Soloway MD &  Brian L. Strom MD, MPH

  3. Clinical Epidemiology Unit, Division of General Medicine of the Department of Medicine, University of Pennsylvania School of Medicine, 221L Nursing Education Building, 19104-6095, Philadelphia, Pennsylvania

    Dimitrios Iliopoulos MD, Barbara Atkinson MD, Scott H. Saul MD, Meenhard Herlyn DVM, Hector A. Rodriguez-Martinez MD, Suzanne L. West MPH, Greg Maislin BS, MA, Roger D. Soloway MD &  Brian L. Strom MD, MPH

  4. the Gastrointestinal Section of the Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania

    Dimitrios Iliopoulos MD, Barbara Atkinson MD, Scott H. Saul MD, Meenhard Herlyn DVM, Hector A. Rodriguez-Martinez MD, Suzanne L. West MPH, Greg Maislin BS, MA, Roger D. Soloway MD &  Brian L. Strom MD, MPH

  5. the Department of Pathology, Hospital General de Mexico y Facultad de Medicina, UNAM, Mexico City, Mexico

    Dimitrios Iliopoulos MD, Barbara Atkinson MD, Scott H. Saul MD, Meenhard Herlyn DVM, Hector A. Rodriguez-Martinez MD, Suzanne L. West MPH, Greg Maislin BS, MA, Roger D. Soloway MD &  Brian L. Strom MD, MPH

Authors
  1. Dimitrios Iliopoulos MD
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  2. Barbara Atkinson MD
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  3. Scott H. Saul MD
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  4. Meenhard Herlyn DVM
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  5. Hector A. Rodriguez-Martinez MD
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  6. Suzanne L. West MPH
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  7. Greg Maislin BS, MA
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  8. Roger D. Soloway MD
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  9. Brian L. Strom MD, MPH
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Additional information

This study was supported by grants CA 35934 and CA 25874 from the National Cancer Institute.

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Iliopoulos, D., Atkinson, B., Saul, S.H. et al. Differences in antigen expression between neoplastic and nonneoplastic gallbladder epithelium. Digest Dis Sci 38, 155–160 (1993). https://doi.org/10.1007/BF01296789

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  • DOI: https://doi.org/10.1007/BF01296789

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