Abstract
Human bile samples are commonly used in biliary research; however, the optimal sampling technique is not known. The current study examines whether bile obtained prior to operative manipulation of the gallbladder differs in composition from samples obtained after cholecystectomy. Gallbladder bile samples were obtained from 26 patients undergoing cholecystectomy. An initial sample was obtained prior to manipulation or devascularization of the gallbladder, and a second sample was obtained after the removal of the gallbladder from the operative field. Gallbladder bile pH and total protein were significantly increased in the postcholecystectomy samples. Bile obtained after cholecystectomy also contained significantly less phospholipid. Gallbladder bile cholesterol, total bile acids, bilirubin, ionized and total calcium, cholesterol saturation index, and total lipids were similar between techniques. These results indicate that manipulation of the gallbladder during cholecystectomy produces alterations in gallbladder bile composition. These results also emphasize the need for consistent sampling technique when obtaining samples for biliary research.
Similar content being viewed by others
References
Strasberg SM, Harvey PRC, Hofmann AF: Bile sampling, processing, and analysis in clinical studies. Hepatology 12:176S-182S, 1990
Janowitz P, Swobodnik W, Wechsler JG, Zoller A, Kuhn K, Ditschunheit H: Comparison of gallbladder bile and endoscopically obtained duodenal bile. Gut 31:1407–1410, 1990
Campbell BA, Burton AC: Stratification of bile in the gallbladder and cholelithiasis. Surg Gynecol Obstet 88:731–738, 1949
Tera H: Stratification of human gallbladder bilein vivo. Acta Chir Scand 256(suppl):4–85, 1960
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254, 1976
Dryer RL, Tammes AR, Routh JI: The determination of phosphorus and phosphatase withN-phenyl-p-phenylenediamine. J Biol Chem 255:177–183, 1956
Roschlau P, Bernt E, Gruber W: Cholesterol and esterified cholesterol. Methods Enzym Anal 4:1890–1893, 1974
Talalay P: Enzymatic analysis of steroid hormones. Methods Biochem Anal 8:119–143, 1960
Carey MC: Critical tables for calculating the cholesterol saturation of native bile. J Lipid Res 19:945–955, 1978
Spivak W, Yeuy W: Application of a rapid and efficient h.p.l.c. method to measure bilirubin and its conjugates from native bile and model bile systems. Biochem J 234:101–109, 1986
Weinman SA, Reuss L: Na+−H+ exchange at the apical mambrane of the nectarus gallbladder: Extracellular and intracellular pH studies. J. Gen Physiol 80:299–321, 1982
Lee SP, LaMont JT, Carey MC: Role of gallbladder mucus hypersecretion in the evolution of cholesterol gallstones. J Clin Invest 67:1712–1723, 1981
Author information
Authors and Affiliations
Additional information
Supported in part by N.I.H. Grant R29-DK41889 from the National Institutes of Diabetes, Digestive, and Kidney Diseases (K.D.L.) and a Veterans Administration Merit Review (H.A.P.)
Rights and permissions
About this article
Cite this article
Ahrendt, S.A., Magnuson, T.H., Pitt, H.A. et al. Effect of cholecystectomy on gallbladder bile composition. Digest Dis Sci 37, 1232–1235 (1992). https://doi.org/10.1007/BF01296565
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01296565