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Digestive Diseases and Sciences

, Volume 37, Issue 5, pp 645–649 | Cite as

Protective effect of 16,16-dimethyl prostaglandin E2 on isolated rat hepatocytes against complement-mediated immune attack

  • Yoichi Kurebayashi
  • Takuya Ikeda
  • Yuko Honda
Original Articles
  • 12 Downloads

Abstract

16,16-Dimethyl prostaglandin E2 was examined for its ability to inhibit complement-mediatedin vitro hepatocytolysis by an antigen-antibody reaction. In the presence of fresh rat serum as a source of complement, 5-min culture of isolated rat hepatocytes with a monoclonal antibody against a rat liver-specific membranous antigen resulted in a marked, significant elevation in lactate dehydrogenase leakage into the culture medium. However, with heat-inactivated rat serum, such a reaction did not occur, indicating that the hepatocytolysis induced by the antibody was attributable to the membrane damaging action of complement activated by an antigen-antibody reaction. Pretreatment of the hepatocyte with 16,16-dimethyl prostaglandin E2 significantly suppressed the cytolytic reaction induced by the antibody in a concentration-dependent manner. These results show that 16,16-dimethyl prostaglandin E2 is capable of protecting isolated rat hepatocytes against the membrane-damaging insult of activated complement.

Key Words

16,16-dimethyl prostaglandin E2 complement antigen-antibody reaction hepatocytolysis cytoprotection 

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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Yoichi Kurebayashi
    • 1
  • Takuya Ikeda
    • 1
  • Yuko Honda
    • 1
  1. 1.Daiichi Pharmaceutical Co., Ltd., 16-13Tokyo 134Japan

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