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Circadian rhythms of gastric mucus efflux and residual mucus gel in the fasting rat stomach

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Abstract

We hypothesized that two putative gastric protective factors, mucus efflux and residual mucus gel content, would manifest circadian rhythms, as reported in several other gastric functions. Rats were adapted for three weeks on a 12-hr light schedule, fasted 18-hr and studied at 3-hr intervals. Under anesthesia, the stomachs were cannulated and filled with test solution. Thirty minutes later, they were drained and the luminal fluid was analyzed for mucus content by Alcian blue binding. Residual mucus gel was determined by direct injection of dye into the lumen. Alcian blue binding of rat mucus was expressed as equivalent milligrams of porcine mucin. Both parameters showed a significant (P<0.001) circadian rhythm. Mucus efflux peaked at 5:03±0:52 HALO (hours after lights on), and residual mucus at 6:00±0.46 HALO. Thus, the interplay of circadian rhythms in aggressive and defensive gastric mucosal functions is supported.

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Authors and Affiliations

  1. Departments of Medicine, and Surgery, Salt Lake Veterans Affairs Medical Center, 84148, Salt Lake City, Utah

    Kenneth R. Larsen PhD, John G. Moore MD & Merril T. Dayton MD

  2. University of Utah School of Medicine, 84112, Salt Lake City, Utah

    Kenneth R. Larsen PhD, John G. Moore MD & Merril T. Dayton MD

Authors
  1. Kenneth R. Larsen PhD
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  2. John G. Moore MD
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  3. Merril T. Dayton MD
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Larsen, K.R., Moore, J.G. & Dayton, M.T. Circadian rhythms of gastric mucus efflux and residual mucus gel in the fasting rat stomach. Digest Dis Sci 36, 1550–1555 (1991). https://doi.org/10.1007/BF01296396

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  • DOI: https://doi.org/10.1007/BF01296396

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