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Modulation of collagen synthesis by fat-storing cells, isolated from CCl4-or vitamin A-treated rats

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Abstract

In an attempt to elucidate the role of fat-storing cells (FSCs) in liver fibrosis, we investigated the collagen synthesis by FSCs freshly isolated from rats treated with CCl4, with vitamin A, and from untreated rats. FSCs from CCl4-treated rats contained a small number of lipid droplets and an abundant rough endoplasmic reticulum (RER), while those from vitamin A-treated rats showed numerous large lipid droplets and scanty RER. The population doubling times of FSCs isolated from normal, CCl4-treated, and vitamin A-treated rats were 38±4.3, 24±2.5, and 48±6.3 hr, respectively. The rate of collagen synthesis by FSCs from CCl4-treated rats was four- to sixfold enhanced, while collagen synthesis by FSCs from vitamin A-treated rats was suppressed. The ratio of collagen type I to type III produced by FSCs from CCl4 rats was enhanced as compared with control rats (94.7:5.3 vs 87.6:12.4). Therefore, FSCs can be considered to play an important role in the pathogenesis of liver fibrosis.

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Authors and Affiliations

  1. Laboratory for Cell Biology and Histology, Faculty of Medicine and Pharmacy, Free University of Brussels (V.U.B.), Laarbeeklaan 103, 1090, Brussels-Jette, Belgium

    Yasushi Shiratori MD, Takafumi Ichida MD, Albert Geerts MD & Eddie Wisse PhD

  2. 2nd Department of Internal Medicine, Faculty, of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan

    Yasushi Shiratori MD, Takafumi Ichida MD, Albert Geerts MD & Eddie Wisse PhD

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  1. Yasushi Shiratori MD
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  2. Takafumi Ichida MD
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  3. Albert Geerts MD
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  4. Eddie Wisse PhD
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Shiratori, Y., Ichida, T., Geerts, A. et al. Modulation of collagen synthesis by fat-storing cells, isolated from CCl4-or vitamin A-treated rats. Digest Dis Sci 32, 1281–1289 (1987). https://doi.org/10.1007/BF01296379

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  • DOI: https://doi.org/10.1007/BF01296379

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