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Interface between haustoria of parasitic members of the Scrophulariaceae and their hosts: A histochemical and immunocytochemical approach

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Summary

The haustorial structure of three African parasitic members of the family Scrophulariaceae (Buchnera hispida, Rhamphicarpa fistulosa, andStriga hermonthica) has been studied with regard to the interface between haustoria and the invaded host roots. Immunocytochemical observations at the light and electron microscopical level were carried out with monoclonal antibodies against pectin. JIM5, JIM7, and hydroxyproline-rich glycoprotein (HRGP), LM1. Lignins have been visualized by phloroglucinolhydrochloric acid staining. At the margin of the lateral interface (contact area of host root cortex and parasite cells), JIM5- and JIM7-labelled substances accumulate between parasite papillae and the host root surface indicating that pectins are implicated in sealing the parasite to the attacked host organ. The lateral interface is characterized by the presence of compressed, necrotic host cells, whereas the central interface (contact area between host stele and parasite cells) is generally devoid of host cell remnants. Phenolic substances and/or lignins can be found at the site of penetration of the haustorium into the host root. These observations and the fact that HRGPs accumulate at the host side of the interface support the view of, at least, a partial defense reaction in the invaded host root tissues. Within haustoria, HRGPs were restricted to differentiating xylem elements, implying a spatio-temporal regulation of HRGPs in developmental processes.

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Abbreviations

BSA:

bovine serum albumin

FITC:

fluorescein isothiocyanate

HRGP:

hydroxyproline-rich glycoprotein

LM:

light microscopy

MAb:

monoclonal antibody

TBSB:

Tris-buffered saline with bovine serum albumin

TBSB-T:

Tris-buffered saline with bovine serum albumin and Tween 20

TEM:

transmission electron microscopy

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Authors and Affiliations

  1. Cytologie Expérimentale et Morphogenèse Végétale, Université Pierre et Marie Curie, 4 place Jussieu, case courrier 150, F-75252, Paris Cedex 05, France

    U. Neumann & G. Sallé

  2. Laboratoire de Pathologie Végétale, Institut National Agronomique Paris-Grignon, Paris

    B. Vian

  3. Morphologie und Systematik der Höheren Pflanzen, Fachbereich Biologie, Philipps-Universität Marburg, Marburg

    H. C. Weber

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  1. U. Neumann
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  2. B. Vian
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  3. H. C. Weber
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  4. G. Sallé
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Neumann, U., Vian, B., Weber, H.C. et al. Interface between haustoria of parasitic members of the Scrophulariaceae and their hosts: A histochemical and immunocytochemical approach. Protoplasma 207, 84–97 (1999). https://doi.org/10.1007/BF01294716

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