Summary
A method to permeabilize theNitella cell without using detergent was developed. The cell was pretreated with an EGTA-containing medium and subsequently plasmolysed at low temperature. The streaming which had been stopped in a medium containing no ATP could be reactivated by the Mg · ATP-containing medium and continued for more than one hour. The rate of the reactivated streaming for the first 30 minutes was almost the same as that of the normal streaming. The reactivated streaming was inhibited irreversibly by Mg2+ depletion and reversibly by cytochalasin B. Thus, the present model is superior to the previous detergent model in which the streaming lasted shorter time and was inhibited irreversibly by cytochalasin B (Shimmen andTazawa 1982a).
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Abbreviations
- ATP:
-
adenosine-5′-triphosphoric acid
- APW:
-
artificial pond water
- CB:
-
cytochalasin B
- CyDTA:
-
1,2-cyclohexanediamine N,N′-tetraacetic acid
- DMSO:
-
dimethylsulfoxide
- EGTA:
-
ethyleneglycol-bis(β-aminoethyl ether)-N,N′-tetraacetic acid
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Shimmen, T., Tazawa, M. Control of cytoplasmic streaming by ATP, Mg2+ and cytochalasin B in permeabilizedCharaceae cell. Protoplasma 115, 18–24 (1983). https://doi.org/10.1007/BF01293576
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DOI: https://doi.org/10.1007/BF01293576