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Rhizogenesis fromNigella sativa protoplasts

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Summary

Protoplasts were isolated for the first time from cell suspensions ofNigella sativa. These were then cultured in media and observed at regular intervals. Different concentrations of auxin and kinetin were tried with success to obtain root from the callus tissues of the protoplasts.

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References

  • Banerjee, S., Gupta, S., 1975: Morphogenesis and tissue culture of different organ ofNigella sativa. Physiol. Plant.33, 185–187.

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  • Cocking, E. C., 1960: A method for the isolation of plant protoplasts and vacuoles. Nature187, 962–963.

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  • Reinert, J., Bajaj, Y. P. S., 1977: Applied and fundamental aspects of plant cell, tissue and organ culture. Berlin-Heidelberg-New York: Springer.

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Authors and Affiliations

  1. Chromosome Research Centre, Department of Botany, University of Calcutta, 700019, Calcutta, India

    T. B. Jha & S. C. Roy

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  1. T. B. Jha
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  2. S. C. Roy
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Jha, T.B., Roy, S.C. Rhizogenesis fromNigella sativa protoplasts. Protoplasma 101, 139–142 (1979). https://doi.org/10.1007/BF01293443

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  • DOI: https://doi.org/10.1007/BF01293443

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