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3H-UTP incorporation in polytene chromosomes ofChironomus permeabilized cells

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Summary

A method is described that allows the cytological detection of the transcriptional activity of endogenous RNA polymerase in fixedChironomus polytene chromosomes.

3H-UTP is preferentially incorporated onto nucleoli and Balbiani rings where transcription is specially high. The former incorporation was sensitive to actinomycin D while incorporation on Balbiani rings was sensitive to α-amanitin. The label pattern and frequency resemble those detected by3H-uridine incorporation in the living state.

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Authors and Affiliations

  1. Instituto de Biología Celular (C.S.I.C.), Velázquez, 144, Madrid-6, Spain

    J. L. Díez & C. de la Torre

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  1. J. L. Díez
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  2. C. de la Torre
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Díez, J.L., de la Torre, C. 3H-UTP incorporation in polytene chromosomes ofChironomus permeabilized cells. Protoplasma 101, 81–87 (1979). https://doi.org/10.1007/BF01293437

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  • DOI: https://doi.org/10.1007/BF01293437

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