Skip to main content
Log in

Saponin distribution in the etiolated leaf tissue and subcellular localization of steroidal saponins in etiolated protoplasts of oat (Avena sativa L.)

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

The present paper describes the saponin distribution in etiolated leaf tissue of oat primary leaves and the subcellular localization of saponins in etiolated oat mesophyll protoplasts. Both avenacosides A and B are predominantely localized in the epidermis of etiolated leaves, in contrast to the epidermis of green leaves, which contains only avenacoside B. Therefore only 30% of the total leaf saponins are found in isolated etiolated mesophyll protoplasts. Evidence is presented that the oat saponins are localized inside the vacuole of etiolated protoplasts as it has already been shown for green protoplasts. Intact etioplasts and prolamellar bodies, isolated via protoplasts, contain only traces or no saponins, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Kesselmeier, J., 1982: Steroidal saponins in etiolated, greening and green leaves and in isolated etioplasts and chloroplasts ofAvena sativa. Protoplasma112, 127–132.

    Google Scholar 

  • —,Laudenbach, U., 1982: Building units of prolamellar bodies: An open question. In: Biochemistry and Metabolism of Plant Lipids (Wintermanns, J. F. G. M., Kuiper, P. J. C., eds.), pp. 585–590. Amsterdam: Elsevier/North Holland Biomedical Press.

    Google Scholar 

  • —,Urban, B., 1983: Subcellular localization of saponins in green and etiolated leaves and green protoplasts of oat (Avena sativa L.). Protoplasma114, 133–140.

    Google Scholar 

  • Lichtenthaler, H. K., Bach, T. J., Wellburn, A. R., 1982: Cytoplasmic and plastidic isoprenoid componds of oat seedlings and their distinct labelling from14C-mevalonate. In: Biochemistry and Metabolism of Plant Lipids (Wintermanns, J. F. G. M., Kuiper, P. J. C., eds.), pp. 489–500. Amsterdam: Elsevier/North Holland Biomedical Press.

    Google Scholar 

  • Murakami, S., Ikeuchi, M., 1982: Biochemical characterization and localization of the 36,000—dalton NADPH: protochlorophyllide oxidoreductase in squash etioplasts. In: Cell Function and Differentiation. Part B (Akoyunoglou, G., ed.), pp. 13–23. New York: Alan R. Liss, Inc.

    Google Scholar 

  • Ryberg, M., Sundqvist, C., 1982: Characterization of prolamellar bodies and prothylakoids fractionated from wheat etioplasts. Physiol. Plant.56, 125–132.

    Google Scholar 

  • —,Sandelius, A. S., Selstam, E., 1983: Lipid composition of prolamellar bodies and prothylakoids fractionated from wheat etioplasts. Physiol. Plant.57, 555–560.

    Google Scholar 

  • Schönbeck, F., Schlösser, E., 1976: Preformed substances as potential protectants. In: Encyclopedia of Plant Physiology, New Series, Vol. 4 (Pirson, A., Zimmermann, M. H., eds.), pp. 653–678. Berlin-Heidelberg-New York: Springer.

    Google Scholar 

  • Verhoek, B.,Haas, R.,Wrage, K.,Linscheid, M.,Heinz, E., 1983: Lipids and enzymatic activities in vacuolar membranes isolated via protoplasts from oat primary leaves. Z. Naturforsch.38c, in press.

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Urban, B., Laudenbach, U. & Kesselmeier, J. Saponin distribution in the etiolated leaf tissue and subcellular localization of steroidal saponins in etiolated protoplasts of oat (Avena sativa L.). Protoplasma 118, 121–123 (1983). https://doi.org/10.1007/BF01293068

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01293068

Keywords

Navigation