Summary
It was investigated whether the low membrane potential at the tip of hyphae ofNeurospora crassa could be described as a diffusion potential. The distribution of Na, K and P inNeurospora was measured by electron microprobe analysis of freeze-dried hyphae. Quantitative determinations were made with freeze-dried gelatin standards and with crystalline standards. The local concentrations of Na and K were in accordance with the theory that at the tip of the hyphe, these ions are distributed passively across the plasma membrane.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dormans, J. A. M. A., 1976: Autoradiografische lokalisatie van Na+-K+-ATPase met 3H-ouabaine. Thesis, Nijmegen.
Hall, T. A., 1975: Methods of quantitative analysis. J. Microscopie Biol. Cell.22, 271–282
Heinrich, K. F. J., 1966: X-ray absorption uncertainty. In: The electron microprobe, pp. 296–377. New York: Wiley.
Martin, P. M., andD. M. Poole, 1971: Electron probe microanalysis: the relation between intensity ratio and concentration. Metallurgical Reviews150, 19–46.
Morgan, A. J., T. W. Davies, andD. A. Erasmus, 1975: Changes in the concentration and distribution of elements during electron. microscope preparative procedures. Micron6, 11–23.
Reed, S. J. B., 1965: Characteristic fluorescence corrections in electron probe microanalysis. Brit. J. appl. Phys.16, 913–926.
Roomans, G. M., andL. A. Sevéus, 1976: Subcellular localization of diffusible ions in the yeastSaccharomyces cerevisiae: quantitative microprobe analysis of thin freeze-dried sections. J. Cell Sci.21, 119–127.
— —, 1977: Preparation of thin cryosectioned standards for quantitative microprobe analysis. J. submicr. Cytol.9, 31–35.
Russ, J. C., 1974: X-ray microanalysis in the biological sciences. J. submicr. Cytol.6, 55–79.
Sevéus, L., 1978: Preparation of biological material for X-ray microanalysis of diffusible elements. 1. Rapid freezing of biological tissue in N2 slush and preparation of ultrathin frozen sections in the absence of trough liquid. J. Microsc., in press.
Slayman, C. L., 1965: Electrical properties ofNeurospora crassa. Effects of external cations on the intracellular potential. J. gen. Physiol.49, 69–92.
—, andC. W. Slayman, 1962: Measurement of membrane potentials inNeurospora. Science136, 876–877.
Theisen, R., 1965: Quantitative electron microprobe analysis. Berlin-Heidelberg-New York: Springer.
Vogel, H. J., 1956: A convenient growth medium forNeurospora (medium N). Microbial Genetics Bull.13, 42–43.
Zs. Nagy, I., C. Pieri, C. Giuli, C. Bertoni-Freddari, andV. Zs. Nagy, 1977: Energydispersive X-ray microanalysis of the electrolytes in biological bulk specimens. I. Specimen preparation, beam penetration and quantitative analysis. J. Ultrastruct. Res.58, 22–33.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Roomans, G.M., Boekestein, A. Distribution of ions inNeurospora crassa determined by quantitative electron microprobe analysis. Protoplasma 95, 385–392 (1978). https://doi.org/10.1007/BF01291413
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01291413