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Kontraktionseigenschaften von isoliertem Schleimpilzactomyosin

1. Vergleichende Untersuchungen an Fadenmodellen aus natürlichen, rekombinierten und hybridisierten Actomyosinen von Schleimpilz und Muskel

Contraction properties of isolated slime mould actomyosin

1. Comparison of thread models made of natural, recombined, and hybridized actomyosins from slime mould and muscle

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Summary

The isolated contractile proteins of the slime mouldPhysarum polycephalum and of rabbit skeletal muscle were investigated by using actomyosin thread models. The actomyosins were compared with respect to contraction behaviour, fine structure, and ATPase activity. Thread models were made of natural and synthetic actomyosins of both systems.

The natural actomyosins differ considerably: The actin filament length ofPhysarum actomyosin is only about one fourth, the ATPase activity and actin/myosin ratio are much lower compared to natural muscle actomyosin. The contraction rate of the natural slime mould actomyosin is remarkably slower than that of the natural muscle actomyosin.

The synthetic actomyosins were formed from separately isolated actins and myosins with a constant actin/myosin ratio and comparable actin filament lengths. The thread models of either recombined and hybridized actomyosins of both systems contract with nearly identical rates. The comparison of the synthetic actomyosins shows that under comparable conditions a) the actomyosins of both systems perform work with the same efficiency, b) the actin and myosin component is freely exchangeable without any change in the rate of actomyosin contraction. These results indicate that in both skeletal muscle and slime mould the force generation is based on the same mechanism of actin-myosin interaction.

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Author notes

  1. J. D'Haese

    Present address: Institut für Zoologie II, Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf, Bundesrepublik Deutschland

Authors and Affiliations

  1. Institut für Cytologie, Universität Bonn, D-5300, Bonn, Bundesrepublik Deutschland

    J. D'Haese & H. Hinssen

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  1. J. D'Haese
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  2. H. Hinssen
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Ein Teil dieser Ergebnisse wurde als Symposiumsvortrag auf dem „9th Meeting of the Federation of the European Biochemical Societies, Budapest“ vorgetragen.

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D'Haese, J., Hinssen, H. Kontraktionseigenschaften von isoliertem Schleimpilzactomyosin. Protoplasma 95, 273–295 (1978). https://doi.org/10.1007/BF01291405

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