Summary
Cytochromec was ionically bound to cell walls as a useful extrinsic marker. The marker binds rapidly, can readily be removed by salt treatment, and can be quantitated by conventional spectrophotometric procedures. The use of the marker allows each step of a cell wall purification to be monitored qualitatively and, furthermore, it provides a rapid, simple quantitative method to determine the yield of purified cell walls once isolated from the cell.
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Abbreviations
- pI:
-
Isoelectric pH
- EDTA:
-
Ethylenediaminetetra-acetic acid
- kD:
-
kiloDaltons
- Cytc:
-
Cytochrome c
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid
- MES:
-
2(N-Morpholino)ethanesulfonic acid
References
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Nagahashi, G., Garzarella, L. An extrinsic marker for higher plant cell walls. Protoplasma 143, 165–169 (1988). https://doi.org/10.1007/BF01291160
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DOI: https://doi.org/10.1007/BF01291160