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Dynamics of the microtubular cytoskeleton in the green algaAphanochaete magna (Chlorophyta). I. Late mitotic stages and the origin and development of the phycoplast

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Summary

The spatial and temporal organization of the microtubular cytoskeleton at the transitional stage of mitosis and cytokinesis has been studied in the chaetophoralean green algaAphanochaete magna using indirect immunofluorescence light microscopy and transmission electron microscopic analysis of serial sections including computer-aided three-dimensional reconstruction. At late mitosis, elaborate asterlike microtubule systems including bundles interconnecting both centriolar regions are present. These systems disappear a the onset of interzonal spindle disintegration. The incipient phycoplast consists of a star-shaped microtubule assemblage projecting from the intact interzonal spindle. It develops strongly at the time of spindle disintegration, later on it becomes compressed by daughter nuclei movement. Cell plate formation is associated with a two-dimensional phycoplast. Phycoplast microtubules remain for a while associated with the completed cross wall but finally they depolymerize. The general occurrence of astral microtubule systems (includingA. magna) is evaluated. The subsequent developmental stages of the phycoplast, formation, maturation and depolymerization, are discussed.

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Abbreviations

IF:

immunofluorescence

IZS:

interzonal spindle

MT:

microtubule

MTOC:

microtubule organizing center

TEM:

transmission electron microscopy

References

  • Aist JR., Berns MW (1981) Mechanics of chromosome separation during mitosis inFusarium (Fungi imperfecti): new evidence from ultrastructural and laser microbeam experiments. J Cell Biol 91: 446–458

    Google Scholar 

  • Bajer A (1968) Fine structure studies on phragmoplast and cell plate formation. Chromosoma 24: 383–417

    Google Scholar 

  • —,Allen RD (1966) Role of phragmoplast filaments in cell-plate formation. J Cell Sci 1: 455–462

    Google Scholar 

  • Bajer AS, Molè-Bajer J (1972) Spindle dynamics and chromosome movements. Int Rev Cytol [Suppl] 3: 1–271

    Google Scholar 

  • Barlow SB, Cattolico RA (1981) Mitosis and cytokinesis in thePrasinophyceae. I.Mantoniella squamata (Manton and Parke) Desikachary. Am J Bot 68: 606–615

    Google Scholar 

  • Bastmeyer M, Steffen W, Fuge H (1986) Immunostaining of spindle components in tipulid spermatocytes using a serum against pericentriolar material. Eur J Cell Biol 42: 305–310

    Google Scholar 

  • Brinkley BR (1985) Microtubule organizing centers. Ann Rev Cell Biol 1: 145–172

    Google Scholar 

  • Deason TR, Darden WH Jr (1971) The male initial and mitosis inVolvox. In:Parker BC, Brown RM Jr (eds) Contributions in phycology. Allen Press, Lawrence, Kansas, pp 67–79

    Google Scholar 

  • Derksen J, Pierson ES, Traas JA (1985) Microtubules in vegetative and generative cells of pollen tubes. Eur J Cell Biol 38: 142–148

    Google Scholar 

  • Domozych DS (1987) Cell division inCarteria crucifera (Chlorophyta): the role of the endomembrane system and phycoplast. Protoplasma 136: 170–182

    Google Scholar 

  • Francke JA, ten Cate HJ (1980) Ecotypic differentiation in response to nutritional factors in the algal genusStigeoclonium Kütz. (Chlorophyceae). Br Phycol J 15: 343-:355

    Google Scholar 

  • Gerlach D (1969) Botanische Mikrotechnik. Eine Einführung. Thieme, Stuttgart

    Google Scholar 

  • Gunning BES (1982) The cytokinetic apparatus: its development and spatial regulation. In:Lloyd CW (ed) The cytoskeleton in plant growth and development. Academic Press, London, pp 229–292

    Google Scholar 

  • Hayat MA (1981) Principles and techniques of electron microscopy. Vol 1: Biological applications, 2nd ed. University Park Press, Baltimore

    Google Scholar 

  • Heath IB, Heath MC (1976) Ultrastructure of mitosis in the cowpea rust fungusUromyces phaseoli var.vignae. J Cell Biol 70: 592–607

    Google Scholar 

  • Hepler PK (1982) Endoplasmic reticulum in the formation of the cell plate and plasmodesmata. Protoplasma 111: 121–133

    Google Scholar 

  • Kilmartin JV, Wright B, Milstein C (1982) Rat monoclonal antitubulin antibodies derived by using a new non-secreting rat cell line. J Cell Biol 93: 576–582

    Google Scholar 

  • Langanger G, de Mey J, Adam H (1983) 1,4-Diazobizyklo-[2.2.2]-ktan (DABCO) verzögert das Ausbleichen von ImmunofluoreszenzprÄparaten. Mikroskopie (Wien) 40: 237–241

    Google Scholar 

  • Lokhorst GM, Barker ME, Star W (1984) Ultrastructure ofDraparnaldia glomerata (Chaetophorales, Chlorophyceae) II. Mitosis and cytokinesis. Nord J Bot 4: 553–562

    Google Scholar 

  • -Sluiman HJ,Star W (in press) Structural and phylogenetic aspects of the ultrastructure of mitosis and cytokinesis inChlorokybus atmophyticus (Chlorophyta, Charophyceae). J Phycol

  • Mattox KR, Stewart KD (1977) Cell division in the scaly green flagellateHeteromastix angulata and its bearing on the origin of theChlorophyceae. Am J Bot 64: 931–945

    Google Scholar 

  • — — (1984) Classification of the green algae: a concept based on comparative cytology. In:Irvine DEG, John DM (eds) Systematics of the green algae. Academic Press, London, pp 29–72

    Google Scholar 

  • McDonald KL, Pfister K, Masuda H, Wordeman L, Staiger C, Cande WZ (1986) Comparison of spindle elongationin vivo andin vitro inStephanopyxis turris. J Cell Sci [Suppl] 5: 205–227

    Google Scholar 

  • Molnar KE, Stewart KD, Mattox KR (1975) Cell division in the filamentousPleurastrum and its comparison with the unicellularPlatymonas (Chlorophyceae). J Phycol 11: 287–296

    Google Scholar 

  • Motomura T, Sakai Y (1985) Ultrastructural studies on nuclear division in the sporophyte ofCarpomitra cabrerae (Clemente) Kützing (Phaeophyta, Sporochnales). Jap J Phycol 33: 199–209

    Google Scholar 

  • Pickett-Heaps JD (1969) The evolution of the mitotic apparatus: an attempt at comparative ultrastructural cytology in dividing plant cells. Cytobios 1: 257–280

    Google Scholar 

  • — (1972) Variations in mitosis and cytokinesis in plant cells: its significance in the phylogeny and evolution of ultrastructural systems. Cytobios 5: 59–77

    Google Scholar 

  • — (1975) Green algae: structure, reproduction and evolution in selected genera. Sinauer Associates, Inc., Sunderland, Massachusetts

    Google Scholar 

  • —,Fowke LC (1970) Mitosis, cytokinesis and cell elongation in the desmidClosterium littorale. J Phycol 6: 189–215

    Google Scholar 

  • —,McDonald K, Tippit DH (1975) Cell division in the pennate diatomDiatoma vulgare. Protoplasma 86: 205–242

    Google Scholar 

  • Schnepf E (1984) Pre- and postmitotic reorientation of microtubule arrays in youngSphagnum leaflets: transitional stages and initiation sites. Protoplasma 120: 100–112

    Google Scholar 

  • Schroeder M, Wehland J, Weber K (1985) Immunofluorescence microscopy of microtubules in plant cells; stabilization by dimethylsulfoxide. Eur J Cell Biol 38: 211–218

    Google Scholar 

  • Segaar PJ, Lokhorst GM (1987) Cell division in the green algaUlothrix palusalsa (Ulvophyceae, Chlorophyta): a combined immunofluorescence and transmission electron microscopy study. Phycologia 26: 100–110

    Google Scholar 

  • Sluiman HJ (1985) Mitosis and cell division inCylindrocapsa geminella (Chlorophyceae). J Phycol 21: 523–532

    Google Scholar 

  • Starr RC (1978) The culture collection of algae at the University of Texas at Austin. J Phycol 14 [Suppl]: 47–100

    Google Scholar 

  • Stewart KD, Mattox KR (1975) Comparative cytology, evolution and classification of the green algae with some consideration of the origin of other organisms with chlorophylls a and b. Bot Rev 41: 104–135

    Google Scholar 

  • — —,Floyd GL (1973) Mitosis, cytokinesis, the distribution of plasmodesmata, and other cytological characteristics in theUlotrichales, Ulvales, andChaetophorales: phylogenetic and taxonomic considerations. J Phycol 9: 128–141

    Google Scholar 

  • Taylor JW (1985) Mitosis in the basidiomycete fungusTulasnella araneosa. Protoplasma 126: 1–18

    Google Scholar 

  • Triemer RE, Brown RM Jr (1974) Cell division inChlamydomonas moewusii. J Phycol 10: 419–433

    Google Scholar 

  • Tupa DD (1974) An investigation of certain chaetophoralean algae. Nova Hedw [Beih] 46: 1–155

    Google Scholar 

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  1. Rijksherbarium, Schelpenkade 6, P.O. Box 9514, NL-2300, RA Leiden, The Netherlands

    P. J. Segaar & G. M. Lokhorst

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  1. P. J. Segaar
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  2. G. M. Lokhorst
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Segaar, P.J., Lokhorst, G.M. Dynamics of the microtubular cytoskeleton in the green algaAphanochaete magna (Chlorophyta). I. Late mitotic stages and the origin and development of the phycoplast. Protoplasma 142, 176–187 (1988). https://doi.org/10.1007/BF01290874

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  • DOI: https://doi.org/10.1007/BF01290874

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