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Suberized bundle sheaths in grasses (Poaceae) of different photosynthetic types. II. Apoplastic permeability

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The relative hydraulic conductivities of major and minor longitudinal veins, and the apoplastic permeability of the bundle sheaths surrounding all longitudinal and transverse veins were investigated in representatives of the C3, C4/NAD-ME, C4/NAD-ME/PCK intermediate, C4/PCK and C4/NADP-ME photosynthetic types. Using the Hagen-Poiseuille equation and measurements of tracheary element diameters, the number of elements in each vein type and the numbers of each vein type, we calculated that 87–99% of the water flow in a longitudinal direction would be expected to occur in the major veins. The permeability of the mestome sheaths and parenchymatous bundle sheaths surrounding the veins was tested using the negatively-charged, fluorescent dye, trisodium 3-hydroxy-5,8,10-pyrenetrisulfonate (PTS). This dye proved nontoxic to plant tissue at a concentration of 0.5%, according to a deplasmolysis test with onion epidermal strips. The PTS concentration achieved in the tested grass leaves was about 0.035%, well below the toxic limit. When a solution of PTS was fed to the leaves by means of a basal cut, the dye moved into the veins of all orders. From there, it moved outward into the surrounding tissues, indicating that the sheaths surrounding the veins of all orders in all species tested were permeable. Therefore, contrary to previous predictions based on structural observations and some tracer studies, bundle sheaths with suberized cell walls do not function as endodermal layers.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Guelph, Guelph, Ontario, Canada

    P. Ann K. Eastman

  2. Department of Biology, University of Waterloo, Waterloo, Ontario, Canada

    Carol A. Peterson

  3. Department of Botany, University of Toronto, M5S 1A1, Toronto, Ontario, Canada

    Nancy G. Dengler

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  1. P. Ann K. Eastman
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  2. Carol A. Peterson
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  3. Nancy G. Dengler
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Eastman, P.A.K., Peterson, C.A. & Dengler, N.G. Suberized bundle sheaths in grasses (Poaceae) of different photosynthetic types. II. Apoplastic permeability. Protoplasma 142, 112–126 (1988). https://doi.org/10.1007/BF01290868

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