Summary
Treatment of long-term tissue cultures of carrots and of adventive embryos in these cultures with “Cellulase Onozuka P 1500” and with this enzyme + “Macerocym” resulted in high yields of protoplasts which could be cultivated over prolonged periods (up to 5 months) in a synthetic medium + 0.6 m sorbitol. During cultivation the size of the protoplasts increased considerably (φ up to 10 times) and a high percentage of multinucleate (between 2 and 13 nuclei) protoplasts were formed. However no fusion and no regeneration of cell walls were observed.
This is a preview of subscription content, log in via an institution to check access.
Literatur
Binding, H., 1966: Regeneration und Verschmelzung nackter Laubmoosprotoplasten. Z. Pflanzenphysiol.55, 305–321.
Chupeau, M. Y., etG. Morel, 1970: Obtention de protoplastes de plantes supérieur à partir de tissus cultivésin vitro. C. R. Acad. Sci. (Paris)270, Ser. D, 2659.
Cocking, E. C., 1960: A method for isolation of plant protoplasts. Nature (London)187, 962–963.
—, 1965 a: Plant protoplasts. Viewpoints in Biology. (J. D. McCarty andC. L. Duddington, eds.). London: Butterworth and Co. Ltd.
Eriksson, T., andK. Jonasson, 1969: Nuclear division in isolated protoplasts from cells of higher plants grownin vitro. Planta (Berl.)89, 85–89.
Gregory, D. W., andE. C. Cocking, 1965: The large-scale isolation of protoplasts from immature tomato fruit. Cell Biology24, 143–146.
Küster, E., 1909: Über die Verschmelzung nackter Protoplasten. Ber. dtsch. bot. Ges.27, 589–598.
Pojnar, E., J. H. M. Willison, andE. C. Cocking, 1967: Cell-wall regeneration by isolated tomato-fruit protoplasts. Protoplasma64, 460–480.
Power, J. B., andE. C. Cocking, 1970: Isolation of leaf protoplasts: Macromolecule uptake and growth substance response. J. Exp. Bot.21, 64–70.
—,S. E. Cummins, andE. C. Cocking, 1970: Fusion of isolated plant protoplasts. Nature225, 1016–1018.
Raj, B., andJ. M. Herr Jr., 1970: The isolation of protoplasts from placental cells ofSolanum nigrum L. Protoplasma69, 291–300.
Schenk, R. U., etA. C. Hildebrandt: Deuxième conference internationale sur les cultures des tissus de plantes. C.N.R.S. Strasbourg, 1970.
Reinert, J.: Factors of embryo formation in plant tissues cultivatedin vitro. Les cultures de tissus de plantes. Coll. Nat. du C.N.R.S. (Paris) 1968.
Ruesink, A. W., andK. V. Thimann, 1965: Protoplasts fromAvenu coleoptile. Proc. nat. Acad. Sci.54, 56–64.
Takebe, I., Otsuki, andS. Aoki, 1968: Isolation of tobacco mesophyll cells in intact and active state. Plant & Cell Physiol.9, 115–124.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hellmann, S., Reinert, J. Protoplasten aus Zellkulturen vonDaucus carota . Protoplasma 72, 479–484 (1971). https://doi.org/10.1007/BF01289516
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01289516