Summary
Germ tube apices ofGilbertella persicaria contain cytoplasmic vesicles, similar to the secretory vesicles found at the tips of vegetative hyphae. The vesicles are present at all stages of development, from the time of germ tube initiation to the establishment of branched hyphae. In contrast to the abundant vesicles at tips of established hyphae, the germ tubes have only a few apical vesicles in a layer next to the plasma membrane. When germinated spores are treated by washing and centrifuging prior to fixation, the cytoplasm is often disrupted near the apex, and the clusters of apical vesicles disappear. The findings indicate the delicate nature of hyphal tips and the necessity of avoiding prefixation stresses in order to preserve the apical apparatus of growing hyphae.
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Akai, S., M. Fukutomi, andH. Kunoh, 1966: An observation on fine structure of conidia ofSphaerotheca pannosa (Wallr.) Lév. attacking leaves of roses. Mycopath. Mycolog. Applicata29, 211–216.
Bartnicki-Garcia, S., N. Nelson, andE. Cota-Robles, 1968 a: A novel apical corpuscle in the hyphae ofMucor rouxii. J. Bacteriol.95, 2399–2402.
— — —, 1968 b: Electron microscopy of spore germination and cell wall formation inMucor rouxii. Arch. Mikrobiol.63, 242–255.
Bonnett, H. T., Jr., andE. H. Newcomb, 1966: Coated vesicles and other cytoplasmic components of growing root hairs of radish. Protoplasma62, 59–75.
Bracker, C. E., 1967: Ultrastructure of fungi. Ann. Rev. Phytopathol.5, 343–374.
—, 1968: The ultrastructure and development of sporangia inGilbertella persicaria. Mycologia60, 1016–1067.
Brenner, D. M., andG. C. Carroll, 1968: Fine-structural correlates of growth in hyphae ofAscodesmis sphaerospora. J. Bacteriol.95, 658–671.
Buckley, P. M., V. E. Sjaholm, andN. F. Sommer, 1966: Electron microscopy ofBotrytis cinerea conidia. J. Bacteriol.91, 2037–2044.
—,N. F. Sommer, andT. T. Matsumoto, 1968: Ultrastructural details in germinating sporangiospores ofRhizopus stolonifer andRhizopus arrhizus. J. Bacteriol.95, 2365–2373.
Butler, E. E., J. M. Ogawa, andT. Shalla, 1960: Notes onGilbertella persicaria from California. Bull. Torrey Bot. Club87, 397–401.
Conti, S. F., andH. B. Naylor, 1960: Electron microscopy of ultrathin sections ofSchizosaccharomyces octosporus. III. Ascosporogenesis, ascospore structure, and germination. J. Bacteriol.79, 417–425.
Franke, W. W., S. Krien, andR. M. Brown, Jr., 1969: Simultaneous glutaraldehyde-osmium tetroxide fixation with postosmication. Histochemie19, 162–164.
Girbardt, M., 1969: Die Ultrastruktur der Apikalregion von Pilzhyphen. Protoplasma67, 413–441.
Grove, S. N., 1971: Protoplasmic correlates of hyphal tip initiation and development in fungi. Ph. D. Thesis, Purdue University, Lafayette, Indiana, U.S.A.
—, andC. E. Bracker, 1970: Protoplasmic organization of hyphal tips among fungi: Vesicles and Spitzenkörper. J. Bacteriol.104, 989–1009.
— —, andD. J. Morré, 1968: Cytomembrane differentiation in the endoplasmic reticulum — Golgi apparatus — vesicle complex. Science161, 171–173.
— — —, 1970: An ultrastructural basis for hyphal tip growth inPythium ultimum. Amer. J. Bot.57, 245–266.
Hashimoto, T., S. F. Conti, andH. B. Naylor, 1958: Fine structure of microorganisms. III. Electron microscopy of resting and germinating ascospores ofSaccharomyces cerevisiae. J. Bacteriol.76, 406–416.
Hawker, L. E., 1966: Germination: morphological and anatomical changes, p. 151–161. In: The Fungus Spore (M. F. Madelin, ed.). London: Butterworth Sci. Publ.
—, andP. McV. Abbott, 1963: An electron microscopic study of maturation and germination of sporangiospores of two species ofRhizopus. J. gen. Microbiol.32, 295–298.
—, andR. J. Hendy, 1963: An electron-microscope study of germination of conidia ofBotrytis cinerea. J. gen. Microbiol.33, 43–46.
—,B. Thomas, andA. Beckett, 1970: An electron microscope study of structure and germination of conidia ofCunninghamella elegans Lenduer. J. gen. Microbiol.60, 181–189.
Heintz, C. E., and D. J.Niederpruem, 1971: Ultrastructure of quiescent and germinated basidiospores and oidia ofCoprinus lagopus. Mycologia63, in press.
Hemmes, D. E., andH. R. Hohl, 1969: Ultrastructural changes in directly germinating sporangia ofPhytophthora parasitica. Amer. J. Bot.56, 300–313.
Hesseltine, C. W., 1960:Gilbertella gen. nov. (Mucorales). Bull. Torrey Bot. Club87, 21–30.
Hyde, J. M., andC. H. Walkinshaw, 1966: Ultrastructure of basidiospores and mycelium ofLenzites saepiaria. J. Bacteriol.92, 1218–1227.
Karnovsky, M. J., 1965: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol.27, 137 A-138 A.
Lowry, R. J., andA. S. Sussman, 1968: Ultrastructural changes during germination of ascospores ofNeurospora tetrasperma. J. gen. Microbiol.51, 403–409.
McClure, W. K., D. Park, andP. M. Robinson, 1968: Apical organization in the somatic hyphae of fungi. J. gen. Microbiol.50, 177–182.
McKeen, W. E., 1970: Lipid inErysiphe graminis hordei and its possible role during germination. Canad. J. Microbiol.16, 1041–1044.
Manocha, M. S., andM. Shaw, 1967: Electron microscopy of uredospores ofMelampsora lini and of rust-infected flax. Canad. J. Bot.45, 1575–1582.
Marchant, R., 1966: Fine structure and spore germination inFusarium culmorum. Ann. Bot.30, 441–445.
Matile, Ph., H. Moor, andC. F. Robinow, 1969: Yeast cytology, p. 219–302. In: The Yeasts. I. Biology of the Yeasts (A.H. Rose andJ. S. Harrison, eds.). London: Academic Press.
Mitchell, N. S., andW. E. McKeen, 1970: Light and electron microscope studies on the conidium and germ tube ofSphaerotheca macularis. Canad. J. Microbiol.16, 273–280.
Moor, H., 1967: Endoplasmic reticulum as the initiator of bud formation in yeast (S. cerevisiae). Arch. Mikrobiol.57, 135–146.
Morré, D. J., H. H. Mollenhauer, andC. E. Bracker, 1970: Origin and continuity of Golgi apparatus, p. 82–126. In: Results and Problems in Cell Differentiation. II. Origin and Continuity of Cell Organelles (T. Reinert andH. Ursprung, eds.). Berlin-Heidelberg-New York: Springer-Verlag.
Remsen, C. C., W. M. Hess, andM. M. A. Sassen, 1967: Fine structure of germinatingPenicillium megasporium conidia. Protoplasma64, 439–451.
Richardson, K. C., L. Jarett, andE. H. Finke, 1960: Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol.35, 313–323.
Sievers, A., 1967: Elektronenmikroskopische Untersuchungen zur geotropischen Reaktion. II. Die polare Organisation des normal wachsenden Rhizoids vonChara foetida. Protoplasma64, 225–253.
Sitte, P., 1963: Zellfeinbau bei Plasmolyse. Protoplasma57, 304–333.
Stocks, D. L., andW. M. Hess, 1970: Ultrastructure of dormant and germinated basidiospores of a species ofPsilocybe. Mycologia62, 176–191.
Sussman, A. S., R. J. Lowry, T. L. Durkee, andR. Maheshwari, 1969: Ultrastructural studies of cold-dormant and germinating uredospores ofPuccinia graminis var.tritici. Canad. J. Bot.47, 2073–2078.
Tanaka, K., 1966: Change in ultrastructure ofAspergillus oryzae conidia during germination. J. gen. appl. Microbiol.12, 239–246.
—, andT. Yanagita, 1963: Electron microscopy on ultrathin sections ofAspergillus niger I. Fine structure of hyphal cells. J. gen. appl. Microbiol.9, 101–118.
Van der Woude, W. J., D. J. Morré, andC. E. Bracker, 1971: Isolation and characterization of secretory vesicles in germinating pollen ofLilium longiflorum. J. Cell Sci.8, 331–351.
Walkinshaw, C. H., J. M. Hyde, andJ. van Zandt, 1967: Fine structure of quiescent and germinating aeciospores ofCronartium fusiforme. J. Bacteriol.94, 245–254.
Williams, P. G., andG. A. Ledingham, 1964: Fine structure of wheat stem rust uredospores. Canad. J. Bot.42, 1503–1508.
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Bracker, C.E. Cytoplasmic vesicles in germinating spores ofGilbertella persicaria . Protoplasma 72, 381–397 (1971). https://doi.org/10.1007/BF01289510
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DOI: https://doi.org/10.1007/BF01289510