Summary
The fine structure of the human cumulus oophorus has been reviewed on the basis of scanning and transmission electron microscopic observations as well as of immunofluorescence data. Tissues sampled from preovulatory ovarian follicles and cumulus-enclosed oocytes and fertilized eggs (collected from the oviduct or obtained during in vitro fertilization procedures) have been evaluated from a microtopographic and morphodynamic point of view in order to better clarify the possible role of this population of cells. In particular, the following aspects have been studied and discussed: the presence of multiple close contacts (modulated by the interposition of the zona pellucida) between the oocyte surface and the long microvillous evaginations projecting from the inner aspect of corona cells surface (through these structures the intraovarian cumulus oophorus may control oocyte growth and metabolism up until the time of ovulation); the occurrence of different subpopulations of cells (steroid-synthetic cells, cells producing adhesive proteins, leukocytes, macrophages) in the postovulatory, extraovarian cumulus oophorus surrounding oocytes, zygotes and early developing embryos. All these elements found in the cumulus mass may positively act, through their paracrine activities, on the chemical composition of the microenvironment in which fertilization occurs.
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References
Carnegie JA, Dardick I, Tsang BK (1987) Microtubules and the gonadotropic regulation of granulosa cell steroidogenesis. Endocrinology 120: 819–828
Chryssikopoulos A (1997) The relationship between immune and endocrine systems. Ann N Y Acad Sci 816: 83–93
Eppig JJ (1982) The relationship between cumulus cell-oocyte coupling, oocyte meiotic maturation and cumulus expansion. Dev Biol 89: 268–272
Familiari G, Franchitto G, Correr S, Motta PM (1979) Microperoxisomes in steroidogenic cells of the rat ovary: interstitial, thecal and luteal cells. Experientia 35: 1503–1505
—, Makabe S, Motta PM (1989) The ovary and the ovulation: a three-dimensional ultrastructural study. In: Van Blerkom J, Motta PM (eds) Ultrastructure of human gametogenesis and early embryogenesis. Kluwer, Boston, pp 85–124
—, Verlengia C, Nottola SA, Renda T, Micara G, Aragona C, Zardi L, Motta PM (1996) Heterogeneous distribution of fibronectin, tenascin-c, and laminin immunoreactive material in the cumulus-corona cells surrounding mature human oocytes from IVFET protocols: evidence that they are composed of different subpopulations: an immunohistochemical study using scanning confocal laser and fluorescence microscopy. Mol Reprod Dev 43: 392–402
Gulyas BJ (1984) Fine structure of the luteal tissue. In: Motta PM (ed) Ultrastructure of endocrine cells and tissues. Martinus Nijhoff, Boston, pp 238–254
Harper MJK (1994) Gamete and zygote transport. In: Knobil E, Neill JD (eds) The physiology of reproduction, 2nd edn. Raven, New York, pp 123–187
Hoshi K, Sasaki H, Yanagida K, Sato A, Tsuiki A (1994) Localization of fibronectin on the surface of human spermatozoa and relation to the sperm-egg interaction. Fertil Steril 61: 542–547
Hutson JC, Garner CW, Doris PA (1996) Purification and characterization of a lipophilic factor from testicular macrophages that stimulates testosterone production by Leydig cells. J Androl 17: 502–508
Larsen WJ, Wert SE, Chen L, Russell P, Hendrix EM (1991) Expansion of the cumulus-oocyte complex during the preovulatory period: possible roles in oocyte maturation, ovulation and fertilization. In: Familiari G, Makabe S, Motta PM (eds) Ultrastructure of the ovary. Kluwer, Boston, pp 45–61.
Macchiarelli G, Vizza E, Nottola SA, Familiari G Nottola SA, Familiari G, Motta PM (1992) Cellular and microvascular changes of the ovarian follicle during folliculogenesis: a scanning electron microscopic study. Arch Histol Cytol 55 Suppl: 191–204
Mahy-Brown CA, Yanagimachi R (1983) Parameters influencing ovum pick-up by oviductal fimbria in the golden hamster. Gamete Res 8: 1–10
Makabe S, Naguro T, Motta PM (1992) A new approach to the study of ovarian follicles by scanning electron microscopy and ODO maceration. Arch Histol Cytol 55 Suppl: 183–190
Mansour RT, Aboulghar MA, Serour GI, Abbass AM (1994) Co-culture of human pronucleate oocytes with their cumulus cells. Hum Reprod 9: 1727–1729
Motta PM, Didio LJA (1974) Microfilaments in granulosa cells during the development of the follicle and its transformation in corpus luteum in the rabbit ovary. J Submicrosc Cytol 6: 15–27
—, Van Blerkom J (1974) A scanning electron microscopic study of the luteo-follicular complex I: follicle and oocyte. J Submicrosc Cytol 6: 297–310
— — (1975) A scanning electron microscopic study of the luteo-follicular complex II: events leading to ovulation. Am J Anat 143: 241–264
—, Takeva Z, Nesci E (1971) Etude ultrastructurale et histochimique des rapports entre les cellules folliculaires et l'ovocyte pendant le développement du follicle ovarien chez les mammifères. Acta Anat 80: 537–562
—, Nottola SA, Familiari G, Macchiarelli G, Vizza E (1992) Follicular structures: an ultrastructural study by scanning and transmission electron microscopy and correlated techniques. In: Sjoberg NO, Hamberger L, Janson PO, Owman Ch, Coeling Bennink HJT (eds) Local regulation of ovarian function. Parthenon, Carnforth, Lanes, pp 39–53
—, Makabe S, Naguro T, Correr S (1994a) Oocyte follicle cells association during development of human ovarian follicle: a study by high resolution scanning and transmission electron microscopy. Arch Histol Cytol 57: 369–394
—, Pereda J, Nottola SA, Familiari G (1994b) Ultrastructural changes of human cumulus oophorus during fertilization and zygote segmentation. In: Mori T, Tominaga T, Aono T, Hiroi M (eds) Perspectives on assisted reproduction: frontiers in endocrinology. Raven, New York, pp 89–95 (Serono Symposia, vol 4)
—, Nottola SA, Pereda J, Croxatto HB, Familiari G (1995a) Ultrastructure of human cumulus oophorus: a transmission electron microscopic study on oviductal oocytes and fertilized eggs. Hum Reprod 10: 2361–2367
—, Familiari G, Macchiarelli G, Vizza E, Correr S (1995b) Ultrastructure of human reproduction from folliculogenesis to early embryo development: a review. Ital J Anat Embryol 100: 9–72
— — — (1997) Cumulus corona cells at fertilization and segmentation: a paracrine organ. In: Motta PM (ed) Microscopy of reproduction and development: a dynamic approach. A Delfino, Roma, pp 173–182
Nottola SA, Familiari G, Micara C, Aragona C, Motta PM (1989) The role of cumulus-corona cells surrounding in vitro human oocytes and polypronuclear ova: an ultrastructural study. Prog Clin Biol Res 296: 345–354
— — — — — (1991) The ultrastructure of human cumulus-corona cells at the time of fertilization and early embryogenesis: a scanning and transmission electron microscopic study in an in vitro fertilization program. Arch Histol Cytol 54: 145–161
Ortiz ME, Salvatierra AM, Lopez J, Fernandez E, Croxatto HB (1982) Postovulatory aging of human ova I: light microscopic observations. Gamete Res 6: 11–17
Path G, Bornstein SR, Ehrhart-Bornstein M, Scherbaum WA (1997) Interleukin-6 and the interleukin-6 receptor in the human adrenal gland: expression and effects on steroidogenesis. J Clin Endocrinol Metab 82: 2343–2349
Rawlins RG, Oronsaye O, Frasor J, Binor Z, Radwanska E (1993) Culture of preimplantation mouse embryo on a reconstituted basement membrane matrix improves early development in vitro. J Assist Reprod Genet Suppl 10: 163
Sathananthan AH, Ng S-C, Bongso A, Trounson A, Ratnam SS (1993) Visual atlas of early human development for assisted reproductive technology. National University of Singapore, Singapore
Shutt DA, Lopata A (1981) The secretion of hormones during the culture of human preimplantation embryos with corona cells. Fertil Steril 35: 413–416
Tesarik J, Dvorak M (1982) Human cumulus oophorus preovulatory development. J Ultrastruct Res 78: 60–72
Wang LJ, Norman RJ (1992) Concentrations of immunoreactive interleukin-1 and interleukin-2 in human preovulatory follicular fluid. Hum Reprod 7: 147–150
—, Pascoe V, Petrucco OM, Norman RJ (1992) Distribution of leukocyte subpopulations in the human corpus luteum. Hum Reprod 7: 197–202
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Motta, P.M., Nottola, S.A., Familiari, G. et al. Structure and function of the human oocyte-cumulus-corona cell complex before and after ovulation. Protoplasma 206, 270–277 (1999). https://doi.org/10.1007/BF01288215
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DOI: https://doi.org/10.1007/BF01288215