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Aberrant nuclear migration and microtubule arrangement in a defect mutant cell ofMicrasterias thomasiana

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Summary

Nuclear migration and the spatial arrangement of the participating microtubules are studied inMicrasterias thomasiana and in the defect mutant cellMicrasterias thomasiana f. uniradiata.

In both of these cell types the two microtubule systems, the “posttelophase system of MT” (PTS) and the “isthmus system of MT” (IS)—which are known to be involved in nuclear migration and anchoring from earlier studies onMicrasterias denticulata—are present in the vicinity of the nucleus. In the mutant cell, however, the orientation of these two MT systems as well as their MT arrangement differ from those in the normalMicrasterias cells. Nuclear migration in the mutant is characterized by a turn of the nucleus and the associated PTS around one of the isthmus invaginations of the cell while in the normalMicrasterias cells it occurs as a straight-lined motion along the longitudinal axis of the cell.

The results indicate that the reduction of cell pattern inMicrasterias caused by mutation is attended by a disoriented establishment of the cytoskeleton involved in nuclear migration. From comparison of the nuclear behavior and the MT arrangement in the mutant with that of the normalMicrasterias cells further information on the mechanism of nuclear migration inMicrasterias is obtained. It is suggested that interactions between the microtubule center (MC), the nuclear envelope and areas of the plasma membrane are functional in the formation, orientation and localization of the nucleus associated microtubule-microfilament complex.

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  1. Institut für Botanik der Universität Salzburg, Lasserstrasse 39, A-5020, Salzburg, Austria

    Ursula Meindl

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Meindl, U. Aberrant nuclear migration and microtubule arrangement in a defect mutant cell ofMicrasterias thomasiana . Protoplasma 126, 74–90 (1985). https://doi.org/10.1007/BF01287675

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