Summary
The development of autofluorescent vacuoles in the stomatal cells ofAllium cepa andA. vineale was investigated using fluorescence microscopy of live cells, low light level television, cytochemistry and electron microscopy. During cell differentiation, the vacuole undergoes two major changes in morphology. In an intermediate form, it consists of a reticulum or network of interlinked tubules and small chambers. The network is formed from globular cisternae in very young GMCs and is maintained as a reticulum until it is transformed back into a globular form later in the differentiation of guard cells. The network thus remains intact through the course of one cell division. During its existence, the reticulum undergoes complex movements and rearrangements. The significance of these changes in the vacuole is discussed in terms of vacuole ontogeny and function and the mechanisms that control motility in plant cells.
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Abbreviations
- DIC:
-
differential interference contrast
- TV:
-
television
- L3TV:
-
low light level television
- GMC:
-
guard mother cell
- I2V:
-
double image intensifier vidicon
- I3V:
-
triple image intensifier vidicon
- CCU:
-
camera control unit
- SEG:
-
special effects generator
- VTR:
-
video tape recorder
- VCR:
-
video cassette recorder
- TDG:
-
time-date generator
- TBC:
-
time base corrector
- SYNC:
-
synchronization
- BL:
-
blanking
- VD:
-
vertical drive
- HD:
-
horizontal drive
- PMT:
-
photomultiplier tube
- ALC:
-
automatic light compensation
- LED:
-
light emitting diode.
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Palevitz, B.A., O'Kane, D.J., Kobres, R.E. et al. The vacuole system in stomatal cells ofAllium. vacuole movements and changes in morphology in differentiating cells as revealed by epifluorescence, video and electron microscopy. Protoplasma 109, 23–55 (1981). https://doi.org/10.1007/BF01287629
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DOI: https://doi.org/10.1007/BF01287629