Summary
Ultrastructural investigations of dormant and germinated sporangiospores ofRhizopus arrhizus were conducted by the use of thin sectioning and freeze-etching procedures. Dormant spores contained deep furrows and prominent ridges with swellings along the sides of the ridges. The furrows and ridges almost disappeared as spores swelled during germination. The plasma membranes contained protuberances or depressions (approximately 50 nm diam) depending upon the nature of the fracture. Mitochondria in dormant spores were spherical and contained few cristae compared to mitochondria of germinated spores which were larger, more diverse in shape and contained abundant cristae. Treatments of spores with 20–25% glycerol prior to freeze-etching or thin sectioning resulted in the production of artifacts between the cell wall and the plasma membrane. Vesicles were rarely observed at the apices of young germ tubes, but were present in abundance in growing hyphal tips. Surface views of young germ tubes revealed the presence of microfibrils.
Similar content being viewed by others
References
Allen, J. V., W. M. Hess, andD. J. Weber, 1971: Ultrastructural investigations of dormantTilletia caries teliospores. Mycologia63, 144–156.
Bracker, C. E., 1971: Cytoplasmic vesicles in germinating spores ofGilbertella persicaria. Protoplasma72, 381–397.
—, andNorma K. Halderson, 1971: Wall fibrils in germinating sporangiospores ofGilbertella persicaria (Mucorales). Arch. Mikrobiol.77, 366–376.
Branton, D., 1966: Fracture faces of frozen membranes. Proc. nat. Acad. Sci.55, 1048–1056.
Buckley, Patricia M., N. F. Sommer, andT. T. Matsumoto, 1968: Ultrastructural details in germinating sporangiospores ofRhizopus stolonifer andRhizopus arrhizus. J. Bacteriol.95, 2365–2373.
Ekundayo, J. A., 1966: Further studies on germination of sporangiospores ofRhizopus arrhizus. J. Gen. Microbiol.42, 283–291.
—, andM. J. Carlile, 1964: The germination of sporangiospores ofRhizopus arrhizus; spore swelling and germ-tube emergence. J. Gen. Microbiol.35, 261–269.
Emmons, C. W., C. H. Binford, andJ. P. Utz, 1970: Medical mycology. pp. 246–248. Philadelphia: Lea & Febiger.
Florance, E. R., W. C. Denison, andT. C. Allen, jr., 1972: Ultrastructure of dormant and germinating conidia ofAspergillus nidulans. Mycologia64, 115–123.
Girbardt, M., 1969: Die Ultrastruktur der Apikalregion von Pilzhyphen. Protoplasma67, 413–441.
Griffiths, D. A., 1971: The fine structure of basidiospores ofPanaeolus campanulatus (L). Fr. revealed by freeze-etching. Arch. Mikrobiol.76, 74–82.
Grove, S. N., andC. E. Bracker, 1970: Protoplasmic organization of hyphal tips among fungi: Vesicles and Spitzenkörper. J. Bacteriol.104, 989–1009.
Hawker, Lilian E., andPatricia McV. Abbott, 1963: An electron microscope study of maturation and germination of sporangiospores of two species ofRhizopus. J. Gen. Microbiol.32, 295–298.
Hemmes, D. E., andH. R. Hohl, 1971: Ultrastructural aspects of encystation and cystgermination inPhytophthora parasitica. J. Cell Sci.9, 175–192.
Hess, W. M., 1966: Fixation and staining of fungus hyphae and host plant root tissue for electron microscopy. Stain Techn.41, 27–35.
—, 1968: Ultrastructural comparisons of fungus hyphal cells using frozen-etched replicas and thin sections of the fungusPyrenochaeta terrestris. Canad. J. Microbiol.14, 205–210.
Hess, W. M., R. L. Bair, andM. Neushul, 1972: Production and cleaning of freeze-etch replicas which show complementary surfaces of fractured fungus spores and hyphae. Stain Techn.47, 249–255.
—, andD. J. Weber, 1972: Freezing artifacts in frozen-etchedRhizopus arrhizus sporangiospores. Mycologia64, 1164–1166.
Moor, H., 1971: Recent progress in the freeze-etching technique. Phil. Trans. Roy. Soc. (Lond.) B.261, 121–131.
Mühlethaler, K., 1971: Studies on freeze-etching of cell membranes. Intern. Rev. Cytol.31, 1–19.
—,H. Moor, andJ. W. Szarkowski, 1965: The ultrastructure of the chloroplast lamellae. Planta (Berl.)67, 305–323.
Nečas, O., M. Havelková, andD. Soudek, 1963: Submicroscopic morphology ofRhizopus nigricans. Folia Microbiol. (Prague)8, 290–292.
Ogawa, J. M., S. D. Lyda, andD. J. Weber, 1961: 2,6-Dichloro-4-nitroaniline effective againstRhizopus fruit rot of sweet cherries. Plant Disease Reporter45, 636–638.
Richmond, D. V., andR. J. Pring, 1971: Fine structure of germinatingBotrytis fabae Sardina conidia. Ann. Bot.35, 493–500.
Sassen, M. M. A., C. C. Remsen, andW. M. Hess, 1967: Fine structure ofPenicillium megasporum conidiospores. Protoplasma64, 75–88.
Sleytr, U., H. Adam undH. Klaushofer, 1969: Die Feinstruktur der Konidien vonAspergillus niger V. Tiegh., dargestellt mit Hilfe der Gefrierätztechnik. Mikroskopie25, 320–331.
Smith, K. O., 1971: Ultrastructural and histochemical investigations ofIpomoea batatus Lam. infected byRhizopus stolonifer (Fr.) Lind. M. S. Thesis. Brigham Young University.
Smith, W. L., Jr., 1962: Chemical treatments to reduce postharvest spoilage of fruits and vegetables. Bot. Rev.28, 411–445.
Stocks, D. L., andW. M. Hess, 1970: Ultrastructure of dormant and germinated basidiospores of a species ofPsilocybe. Mycologia62, 176–191.
Weber, D. J., andJ. M. Ogawa, 1965 a: The mode of action of 2,6-Dichloro-4-nitroaniline inRhizopus arrhizus. Phytopathology55, 159–165.
— —, 1965 b: The specificity of proline in the germination of spores ofRhizopus arrhizus. Phytopathology55, 262–266.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hess, W.M., Weber, D.J. Ultrastructure of dormant and germinated sporangiospores ofRhizopus arrhizus . Protoplasma 77, 15–33 (1973). https://doi.org/10.1007/BF01287290
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01287290