Summary
Considerable transient membrane modifications at the ultrastructural level occurred during the first hours of thein vitro culture ofDatura innoxia leaf explants. Pinocytosis, loosening, outgrowths and folds of plasmalemma, plastid membrane invaginations and continuities between the outer layer of the plastid membrane and the plasmalemma were induced when sucrose was added to the medium. These changes were directly connected with the strong amylogenesis that took place from the 12th to the 72th hour. Kinetin and, to a lesser degree, auxin, magnified the phenomenon. Without sucrose, neither membrane revising nor amylogenesis occurred. Relations between these structural membrane modifications and possible alterations in the cell permeability before the induction of dedifferentiation were discussed.
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This work was aided by grants from the C. N. R. S. (E. R. A. n∘ 616).
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Brossard-Chriqui, D., Iskander, S. Ultrastructural changes of cytomembranes during the first hours of thein vitro culture ofDatura innoxia mill. Leaf explants. Relations to sucrose uptake. Protoplasma 112, 217–225 (1982). https://doi.org/10.1007/BF01284096
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DOI: https://doi.org/10.1007/BF01284096