Summary
Selected nuclear and cytoplasmic changes associated with early differentiation of four cell-types—dermatogen, inner and outer cortex, and endodermis—have been analysed using montages of electron micrographs of median longitudinal sections of young roots ofAzolla pinnata. The area fraction of nucleoplasm occupied by chromocentres (CAF) is smaller in the apical cell than in the nuclei of its most recently formed daughter cells. The CAF also differs between the four cell-types: dermatogen nuclei have a lesser mean CAF and smaller chromocentres than nuclei of the endodermis; cortical cell nuclei have intermediate values. These differences may reflect changes in nuclear activity during cell differentiation. The area fraction occupied by the vacuome (VAF) differs between the apical cell and its daughters: the apical cell seems to retain most of the vacuome at division, while the daughter cells receive less vacuolate cytoplasm. Of the four cell-types analysed, the cortical cells develop a large VAF the quickest; the dermatogen is slower to become vacuolate. Cells in the dermatogen and outer cortex derive from common mother cells, as do cells in the endodermis and inner cortex, and even the most recently-formed cells in the files of inner and outer cortex are more vacuolated than their sister cells in the other two celltypes. The onset of vacuolation may be triggered by an inductive influence emanating from older vacuolated cells in the same file. The rate of vacuolation in each of the cell-types examined may also be negatively correlated to the intensity of synthesis of protein used to construct cytoplasmic materials.
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References
Ashley, T., 1972: Zygote shrinkage and subsequent development in someHibiscus hybrids. Planta108, 303–317.
Barlow, P. W., 1976: The relationship of the dispersion phase of chromocentric nuclei in the mitotic cycle to DNA synthesis. Protoplasma90, 381–392.
—, 1977: The dynamic aspect of cytodifferentiation in cells. Naturwissenschaften64, 532–533.
Chaly, N. M., Setterfield, G., 1975: Organization of the nucleus, nucleolus, and protein synthesizing apparatus in relation to cell development in roots ofPisum sativum. Canad. J. Bot.53, 200–218.
Fineran, B. A., 1970: An evaluation of the form of vacuoles in thin sections and freeze-etch replicas of root tips. Protoplasma70, 457–478.
—, 1971: Effect of various factors of fixation on the ultrastructural preservation of vacuoles in root tips. La Cellule58, 269–286.
Frenster, J. H., 1974: Ultrastructure and function of heterochromatin and euchromatin. In: The Cell Nucleus (Busch, H., ed.). Vol. 1, pp. 565–580. New York-London: Academic Press.
Gunning, B. E. S., Hughes, J. E., Hardham, A. R., 1978: Formative and proliferative cell divisions, cell differentiation, and developmental changes in the meristem ofAzolla roots. Planta143, 121–144.
Gurdon, J. B., Laskey, R. A., Derobertis, E. M., Partington, G. A., 1979: Reprogramming of transplanted nuclei in amphibians. Int. Rev. Cytol., Supplement9, 161–178.
Harris, H., 1970: Cell Fusion. Oxford: Clarendon Press.
Hébant, C, Hébant-Mauri, R., Barthonnet, J., 1978: Evidence for division and polarity in apical cells of bryophytes and pteridophytes. Planta138, 49–52.
Jensen, W. A., 1968: Cotton embryogenesis: the zygote. Planta79, 346–366.
Juniper, B. E., Clowes, F. A. L., 1965: Cytoplasmic organelles and cell growth in root caps. Nature208, 864–865.
Kononowicz, A. K., Kuran, H., Olszewska, M. J., 1976: Cytochemical and ultrastructural studies of changes in the nucleus and nucleolus during cell differentiation in the root cortex ofHaemanthus katherinae. Folia Histochem. Cytochem.14, 151–164.
Lang, A., 1965: Progressiveness and contagiousness in plant differentiation and development. In: Encyclopaedia of Plant Physiology (Ruhland, W., ed.), Vol. 15/1, pp. 409–423. Berlin-Heidelberg-New York: Springer.
Lang, A., 1974: Inductive phenomena in plant development. Brookhaven Symp. Biol.25, 129–144.
Leech, J. H., Mollenhauer, H. H., Whaley, W. G., 1963: Ultrastructural changes in the root apex. Symp. Soc. exp. Biol.17, 74–84.
Matile, P., 1975: The Lytic Compartment of Plant Cells. (Cell Biology Monographs, Vol. 1.) Wien-New York: Springer.
Nagl, W., 1979: Condensed interphase chromatin in plant and animal cell nuclei: fundamental differences. Plant Syst. Evol., Supplement2, 247–260.
O'brien, T. P., Kuo, J., McCully, M. E., Zee, S.-Y., 1973: Coagulant and non-coagulant fixation of plant cells. Austral. J. biol. Sci.26, 1231–1250.
Olszewska, M. J., 1976: Autoradiographic and ultrastructural study ofCucurbita pepo root cells during their growth and differentiation. Histochemistry49, 157–175.
Owens, T., Poole, R. J., 1979: Regulation of cytoplasmic and vacuolar volumes by plant cells in suspension culture. Plant Physiol.64, 900–904.
Poux, N., Favard, P., Carrasso, N., 1974: Etude en microscopie électronique haute tension de l'appareil vacuolaire dans les cellules méristèmatiques de racines de concombre. J. Microscop.21, 173–180.
Schmalenberger, B., 1980: Differentiation of the guinea-pig eye: nuclear ultrastructure, template activity and DNA content. Protoplasma103, 377–391.
Whatley, J. M., Gunning, B. E. S., 1981: Chloroplast development inAzolla roots. New Phytol.89, 129–138.
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Barlow, P.W., Rost, T.L. & Gunning, B.E.S. Nuclear and cytoplasmic changes during early stages of cell differentiation in roots of the water-fern,Azolla pinnata . Protoplasma 112, 205–216 (1982). https://doi.org/10.1007/BF01284095
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DOI: https://doi.org/10.1007/BF01284095