Summary
The distribution of latent UDPase activity (cold storage-activated) is similar to Triton-stimulated UDPase activity in membrane fractions separated by differential centrifugation as well as fractions purified by linear sucrose density centrifugation. The Triton-stimulated UDPase activity appears to be a specific marker for Golgi membranes in corn root homogenates. Detergent-activated UDPase activity provides a more reliable, less cumbersome way to monitor Golgi membranes compared to cold storage-activation and this marker can be used on fresh preparations.
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References
Coughlan, S., Evans, L. V., 1978: Isolation and characterization of Golgi bodies from vegetative tissue of brown algaFucus serratus. J. exp. Bot.29, 55–68.
Dauwalder, M., Whaley, W. G., Kephart, J. E., 1969: Phosphatases and differentiation of the Golgi apparatus. J. Cell. Sci.4, 455–497.
Dulley, J. R., 1975: Determination of inorganic phosphate in the presence of detergents or protein. Anal. Biochem.67, 91–96.
Fiske, C. H., Subbarow, Y., 1925: The colorimetric determination of phosphorus. J. biol. Chem.66, 375–400.
Hartmann-Bouillon, M.-A., Benveniste, P. 1978: Sterol biosynthetic capability of purified membrane fractions from maize coleoptiles. Phytochem.17, 1037–1042.
Hartman-Bouillon, M.-A., Benveniste, P., Roland, J. C., 1979: Sterol metabolism in purified membrane fractions from maize coleoptiles. Biol. cellulaire35, 183–194.
Hodges, T. K., Leonard, R. T., 1974: Purification of a plasma membrane-bound adenosine triphosphatase from plant roots. Methods in Enzymol.32, 392–406. Academic Press, U.S.A.
Klohs, W. D., Goff, C. W., 1973: A characterization of nucleoside diphosphatase in the onion root tip. J. Histochem. Cytochem.21, 417–425.
Klohs, W. D., Goff, C. W., 1980: Characterization of nucleoside diphosphatase in the onion root tip. III. Solubilization and activation of membrane-bound enzyme(s). Eur.J. Cell Biol.20, 228–233.
Koehler, D. E., Leonhard, R. T., Vanderwoude, W. J., Linkins, A. E., Lewis, L. N., 1976: Association of latent cellulase activity with plasma membranes from kidney bean abscission zones. Plant Physiol.58, 324–330.
Kreibich, G., Sabatini, D. D., 1974: Procedure for the selective release of content from microsomal vesicles without membrane disassembly. Methods in Enzymology31, 215–225. Academic Press, U.S.A.
Kuhn, N. J., White, A., 1977: The role of nucleoside diphosphatase in a uridine nucleotide cycle associated with lactose synthesis in rat mammary-gland Golgi apparatus. Biochem. J.168, 423–433.
Leonard, R. T., Hansen, D., Hodges, T. K., 1973: Membrane-bound adenosine triphosphatase activities of oat roots. Plant Physiol.51, 749–754.
Leonard, R. T., Hanson, J. B., 1972: Increased membranebound adenosine triphosphatase activity accompanying development of enhanced solute uptake in washed corn root tissue. Plant Physiol.49, 436–440.
Leonard, R. T., Vanderwoude, W. J., 1976: Isolation of plasma membranes from corn roots by sucrose density gradient centrifugation. An anomalous effect of Ficoll. Plant Physiol.57, 105–114.
Lord, J. M., Kagawa, T., Moore, T. S., Beevers, H., 1973: Endoplasmic reticulum as the site of lecithin formation in castor bean endosperm. J. Cell Biol.57, 659–667.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J., 1951: Protein measurement with the folin phenol reagent. J. biol. Chem.193, 265–275.
Meyer, H. U., Biehl, B., 1981: Activation of latent phenolase during spinach leaf senescence. Phytochem.20, 955–959.
Morrè, D. J., Lembi, C. A., Vanderwoude, W. J., 1977: A latent inosine-5′-diphosphatase associated with Golgi apparatus-rich fractions from onion stem. Cytobiologie16, 72–81.
M'voula-Tsieri, M., Hartmann-Bouillon, M.-A., Benveniste, P., 1981: Properties of nucleoside diphosphatases in purified membrane fractions from maize coleoptiles I. Study of latency. Plant Sci. Letts.20, 379–386.
Nagahashi, J., Beevers, L., 1978: Subcellular localization of glycosyl transferases in glycoprotein biosynthesis in the cotyledons ofPisum sativum L. Plant Physiol.16, 451–459.
Nagahashi, J., Nagahashi, S. L., 1982: Triton-stimulated nucleoside diphosphatase: characterization. Protoplasma112, 174–180.
Peterson, G. L., 1978: A simplified method for analysis inorganic phosphate in the presence of interfering substances. Anal. Biochem.84, 164–172.
Quail, P. H., 1979: Plant cell fractionation. Ann. Rev. Plant Physiol.30, 425–484.
Ray, P. M., Shininger, T. L., Ray, M. M., 1969: Isolation of βglucan synthetase particles from plant cells and identification with Golgi membranes. Proc. Nat. Acad. Sci.64, 605–611.
Wagner, G. J., 1981: Enzymic and protein character of tonoplast fromHippeastrum vacuoles. Plant Physiol.68, 499–503.
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This research was supported in part by NSF grant CDP-7927121 and funds received from the Bronfman Science Center, Williams College.
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Nagahashi, J., Kane, A.P. Triton-stimulated nucleoside diphosphatase activity: Subcellular localization in corn root homogenates. Protoplasma 112, 167–173 (1982). https://doi.org/10.1007/BF01284090
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DOI: https://doi.org/10.1007/BF01284090