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The composition of the spikes ofAcanthosphaera zachariasi (Chlorococcales): Investigations with fluorescent lectins and with X-ray diffraction

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Summary

The spikes ofAcanthosphaera zachariasi (Chlorococcales) were tested for lectin binding, staining reactions, solubility in trifluoroacetic acid (TFA) and with X-ray diffraction. The matrix stains only with Concanavalin A, not with different other lectins. The TFA resistant part is shown in negative stain to consist of ribbon-like fibrils with kinking sites. The spikes reveal a cellulose I-like X-ray diffraction pattern. It is concluded that they consist of cellulose associated with non-cellulosic matrix components.

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Authors and Affiliations

  1. Zellenlehre, University of Heidelberg, Im Neuenheimer Feld 230, D-6900, Heidelberg, Federal Republic of Germany

    W. Herth & E. Schnepf

  2. Institut für Allgemeine Botanik, University of Hamburg, Federal Republic of Germany

    B. Surek

Authors
  1. W. Herth
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  2. E. Schnepf
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  3. B. Surek
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Herth, W., Schnepf, E. & Surek, B. The composition of the spikes ofAcanthosphaera zachariasi (Chlorococcales): Investigations with fluorescent lectins and with X-ray diffraction. Protoplasma 110, 196–202 (1982). https://doi.org/10.1007/BF01283322

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  • DOI: https://doi.org/10.1007/BF01283322

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