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Variation of cell cycle duration within suspension cultures ofDaucus carota and its consequence for the induction of ploidy changes with colchicine

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Summary

Continuous exposure to colchicine was used to estimate the variation in cell cycle time between cells within suspension cultures ofDaucus carota. Observations were made of the pattern of disappearance of cells of the initially predominant ploidy levels in diploid and tetraploid cultures having markedly different aggregation patterns. Both cultures showed a similar range of cycle times, normally distributed about the culture mean. Shorter colchicine treatments, followed by regrowth in colchicine-free medium, showed that spread of cycle times in the diploid culture prevented uniform induction of tetraploidy, and that the resulting mixoploid suspensions showed a gradual reversion to diploidy during subsequent subculture.

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References

  • Bayliss, M. W., 1975 a: The duration of the cell cycle ofDaucus carota in vivo andin vitro. Exp. Cell Res.92 (1), 31–38.

    Article  CAS  PubMed  Google Scholar 

  • —, 1975 a: The effects of growthin vitro on the chromosome complement ofDaucus carota L. suspension cultures. Chromosoma51, 401–411.

    Article  Google Scholar 

  • Gould, A. R., M. W. Bayliss, andH. E. Street, 1974: Studies on the growth in culture of plant cells. XVII. Analysis of the cell cycle of asynchronously dividingAcer pseudoplatanus L. cells in suspension culture. J. exp. Bot.25 (85), 468–478.

    Article  CAS  Google Scholar 

  • King, P. J., and H. E.Street: Growth patterns in cell cultures. In: Plant tissue and cell culture, pp. 269–338 (H. E.Street, ed.). Oxford: Blackwell.

  • Mansfield, K. J., 1973: The isolation of clones by single cell plating. MSc thesis, University of Sheffield.

  • Moore, P. G., E. A. Shirley, andD. E. Edwards, 1972: Standard statistical calculations. London: Pitman.

    Google Scholar 

  • Smith, S. M., andH. E. Street, 1974: The decline of embryogenic potential as callus and suspension cultures of carrot (Daucus carota) are serially subcultured. Ann. Bot.38, 223–241.

    Google Scholar 

  • Van't Hof, J., 1965: Cell population kinetics of excised roots ofPisum sativum, J. Cell Biol.27, 179–189.

    Article  PubMed  PubMed Central  Google Scholar 

  • Webster, P. L., andD. Davidson, 1969: Changes in the duration of the mitotic cycle induced by Colchicine and IAA inVicia faba roots. J. exp. Bot.20, 671–685.

    Article  CAS  Google Scholar 

  • Wilson, S. B., P. J. King, andH. E. Street, 1971: Studies on the growth and culture of plant cells. XII. A versatile system for the large scale batch or continuous culture of plant cell suspensions. J. exp. Bot.21, 177–207.

    Article  Google Scholar 

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  1. M. W. Bayliss

    Present address: Botanical Laboratories, University of Leicester, Leicester, UK

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    1. M. W. Bayliss
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    Bayliss, M.W. Variation of cell cycle duration within suspension cultures ofDaucus carota and its consequence for the induction of ploidy changes with colchicine. Protoplasma 88, 279–285 (1976). https://doi.org/10.1007/BF01283252

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    • DOI: https://doi.org/10.1007/BF01283252

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