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Plasma-membrane-bound malate dehydrogenase activity in maize roots

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Summary

Plasma membranes were isolated and purified from 14-day-old maize roots (Zea mays L.) by two-phase partitioning at a 6.5% polymer concentration, and compared to isolated mitochondria, microsomes, and soluble fraction. Marker enzyme analysis demonstrated that the plasma membranes were devoid of cytoplasmic, mitochondrial, tonoplast, and endoplasmic-reticulum contaminations. Isolated plasma membranes exhibited malate dehydrogenase activity, catalyzing NADH-dependent reduction of oxaloacetate as well as NAD+-dependent malate oxidation. Malate dehydrogenase activity was resistant to osmotic shock, freeze-thaw treatment, and salt washing and stimulated by solubilization with Triton X-100, indicating that the enzyme is tightly bound to the plasma membrane. Malate dehydrogenase activity was highly specific to NAD+ and NADH. The enzyme exhibited a high degree of latency in both right-side-out (80%) and inside-out (70%) vesicle preparations. Kinetic and regulatory properties with ATP and Pi, as well as pH dependence of plasma-membrane-bound malate dehydrogenase were different from mitochondrial and soluble malate dehydrogenases. Starch gel electrophoresis revealed a characteristic isozyme form present in the plasma membrane isolate, but not present in the soluble, mitochondrial, and microsomal fractions. The results presented show that purified plasma membranes isolated from maize roots contain a tightly associated malate dehydrogenase, having properties different from mitochondrial and soluble malate dehydrogenases.

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Abbreviations

FCR:

ferricyanide reductase

MDH:

malate dehydrogenase

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Authors and Affiliations

  1. Maize Research Institute, P.O. Box 89, 11081, Belgrade-Zenum, Yugoslavia

    V. Hadži-Taskovišković, M. Vuletić & D. Ignjatović-Micić

  2. Center for Multidisciplinary Studies, Belgrade University, Belgrade

    Ž. Vučinić

Authors
  1. V. Hadži-Taskovišković
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  2. M. Vuletić
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  3. D. Ignjatović-Micić
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  4. Ž. Vučinić
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Hadži-Taskovišković, V., Vuletić, M., Ignjatović-Micić, D. et al. Plasma-membrane-bound malate dehydrogenase activity in maize roots. Protoplasma 207, 203–212 (1999). https://doi.org/10.1007/BF01283001

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  • DOI: https://doi.org/10.1007/BF01283001

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