Summary
Protoplasts derived from cells ofBoergesenia forbesii regenerated aberrant cell walls when treated with cholesteryl hemisuccinate (CHS). Protoplasts treated with CHS, for a short period during the initial stages of cell wall regeneration, developed a patchwork cell wall, possessing regions devoid of cell wall. This effect was reversible, and treated cells ultimately developed a normal, confluent cell wall when removed from the CHS. Freeze fracture studies revealed that for CHS-treated cells, regions without microfibril impressions did possess intramembranous particles (IMP's) but that these regions contained small domains free of IMP's suggestive of lateral phase separation. The data implies that the physical characteristics of the plasma membrane lipid are important to the deposition of cell wall microfibrils during cell wall regeneration. This effect may be attributed to altered lipid-protein interactions, modified membrane fusion characteristics, or altered membrane flow.
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References
Borochov H, Abbot RE, Schachter D, Shinitzky M (1979) Modulation of erythrocytic membrane proteins by membrane cholesterol and lipid fluidity. Biochemistry 18: 251–255
Itoh T, O'Neil RM, Brown RM, Jr (1984) Interference of cell wall regeneration ofBoergeseniaforbesii protoplasts by Tinopal LPW, a fluorescent brightening agent. Protoplasma 123: 174–183
—,Legge RL, Brown RM, Jr (1986) The effects of selected inhibitors on cellulose microfibril assembly inBoergesenia forbesii (Chlorophyta) protoplasts. J Phycol 22: 224–233
Kudlicka K, Wardrop A, Itoh T, Brown RM, Jr (1987) Further evidence from sectioned material in support of the existence of a linear terminal complex in cellulose synthesis. Protoplasma 135: 96–103
Mizuta S (1985) Evidence for the regulation of the shift in cellulose microfibril orientation in freeze-fractured plasma membrane ofBoergesenia forbesii. Plant Cell Physiol 26: 53–62
Mueller SC, Brown RM, Jr (1982) The control of cellulose microfibril deposition in the cell wall of higher plants. Planta 154: 489–500
O'Neil RM, LaClaire II JW (1984) Mechanical wounding induces the formation of extensive coated membranes in giant algal cells. Science 225: 331–333
Platt-Aloia KA, Thomson WW (1987) Freeze fracture evidence for lateral separations in the plasmalemma of chilling-injured avocado fruit. Protoplasma 136: 71–80
Provasoli L, McLaughlin JJA, Droop MR (1957) The development of artificial media for marine algae. Arch Mikrobiol 25: 392–428
Shinitzky M, Rivnay B (1977) Degree of exposure of membrane proteins determined by fluorescent quenching. Biochemistry 16: 982–986
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Legge, R.L., Brown, R.M. Modification of protoplast cell wall regeneration by membrane perturbation. Protoplasma 143, 38–42 (1988). https://doi.org/10.1007/BF01282957
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DOI: https://doi.org/10.1007/BF01282957