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Profilin is localized in the nucleus-associated microtubule and actin system and is evenly distributed in the cytoplasm of the green algaMicrasterias denticulata

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Summary

Profilin is detected by means of immunoblotting in the green algaMicrasterias denticulata at a molecular mass of about 14 kDa with antibodies against celery root profilin, recombinant tobacco profilin, and recombinant birch profilin. Poly-L-proline purification ofM. denticulata extracts leads to a single band at 14 kDa. By means of immunoelectron microscopy first evidence is provided for the presence of profilin in a microtubule center associated with the migrating nucleus in high-pressure-frozen and freeze-substituted cells. Colocalization with a particular filamen-tous-actin aggregation in the same area suggests a role of profilin in the process of nuclear migration. Moreover staining is visible in several areas of the nucleus including the nucleolus, heterochromatin, and nuclear-pore complexes. An even distribution of profilin is found throughout the cytoplasm at the confocal-microscopy level as well as by immunogold labeling. Occasionally also areas at the surface of the chloroplast are stained.

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Abbreviations

FITC:

fluorescein isothiocyanate

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Authors and Affiliations

  1. Institut für Pflanzenphysiologie, Universität Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria

    A. Holzinger & U. Lütz-Meindl

  2. Institute of General and Experimental Pathology, General Hospital, University of Vienna, Vienna

    R. Valenta

Authors
  1. A. Holzinger
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  2. R. Valenta
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  3. U. Lütz-Meindl
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Correspondence to A. Holzinger.

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Dedicated to Professor Walter Gustav Url on the occasion of his 70th birthday

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Holzinger, A., Valenta, R. & Lütz-Meindl, U. Profilin is localized in the nucleus-associated microtubule and actin system and is evenly distributed in the cytoplasm of the green algaMicrasterias denticulata . Protoplasma 212, 197–205 (2000). https://doi.org/10.1007/BF01282920

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  • DOI: https://doi.org/10.1007/BF01282920

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