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Nuclear crystals, lampbrush-chromosome-like structures, and perinuclear cytoskeletal elements associated with nuclear fragmentation in characean internodal cells

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Summary

This article gives a survey of nucleus-associated structures and inclusions in a diverse range of characean algae includingChara braunii Gm.,Chara corallina Klein ex Willd., em. R.D.W.,Nitella cristata A.Br., em. R.D.W.,Nitella flexilis (L.) Ag.,Nitella furcata (Roxb. ex Bruz.) Ag. em. R.D.W.,Nitella hyalina (DC.) Ag.,Nitella pseudoflabellata A.Br., em. R.D.W.,Nitella pseudoflabellata var.imperialis T.F.A.,Nitella translucens var.axillaris (A.Br.) R.D.W. andNitellopsis obtusa (Desv. in Lois.) J.Gr. Lampbrushchromosome-like structures were found in nuclei ofNitella flexilis andNitellopsis obtusa and seem to be involved in the distribution of genetic material during nuclear fragmentation. Intranuclear tubular crystals of unknown protein composition were present in all species, especially in young, elongating cells, and could be important for establishing the main axis of the nuclei. Spindle-shaped protein crystals that originate in the nucleus and are released into the cytoplasm upon nuclear degeneration were observed in branchlet internodal cells of one population ofNitella flexilis. Perinuclear microtubules were present in all species, but perinuclear actin fibrils were hitherto only found in mostNitella species and inNitellopsis obtusa. None of these nucleus-associated structures seems to be responsible for the formation of constrictions leading to nuclear fragmentation. These constrictions were perpendicular to the main axis of the nucleus and symmetrical in theNitella species but asymmetric inC. braunii, C. corallina, and inNitellopsis obtusa. Statistical analysis of nuclear size, number and constriction sites indicate that fragmentation is a nonsynchronous process independent of the light-dark cycle.

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Abbreviations

CLSM:

confocal laser scanning microscopy

DAPI:

4′,6-diamidino-2-phenylindole

DIC:

Nomarski differential interference contrast

LCLS:

lampbrush chromosome-like structure(s)

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Authors and Affiliations

  1. Institut für Pflanzenphysiologie, Universität Salzburg, Hellbrunnerstrasse 34, A-5020, Salzburg, Austria

    I. Foissner

  2. Plant Cell Biology Group, Research School of Biological Sciences, Australian National University, Canberra, A.C.T.

    G. O. Wasteneys

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  1. I. Foissner
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  2. G. O. Wasteneys
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Dedicated to Professor Walter Gustav Url on the occasion of his 70th birthday

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Foissner, I., Wasteneys, G.O. Nuclear crystals, lampbrush-chromosome-like structures, and perinuclear cytoskeletal elements associated with nuclear fragmentation in characean internodal cells. Protoplasma 212, 146–161 (2000). https://doi.org/10.1007/BF01282916

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  • DOI: https://doi.org/10.1007/BF01282916

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