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Physiological properties and cytological features of protoplasts prepared fromChlorella saccharophila

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Summary

Protoplasts were prepared from cells ofChlorella saccharophila by treatment with a mixture of pectinase and cellulase. The yield of protoplasts is dependent upon the culture conditions prior to cell wall digestion. In thin section chemically-fixed protoplasts were without wall remnants at the surface of the plasma membrane. Of particular interest is the relationship between the Golgi apparatus and a nuclear envelope-endoplasmic reticulum continuum. Protoplasts have a photosynthetic capacity lying between 70 and 80% of that of normal cells, but show the same response towards CO2 concentration and DCMU inhibition. Protoplasts also respond to changes in the osmolarity of the surrounding medium in accordance with the Boylevan't Hoff equation as if they are an osmometer. The nonosmotic volume (NOV) was calculated.

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Abbreviations

GA:

Golgi apparatus

ER:

endoplasmic reticulum

NE:

nuclear envelope

PM:

plasma membrane

N:

nucleus

S:

starch

M:

mitochondria

V:

vacuole

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Authors and Affiliations

  1. Pflanzenphysiologisches Institut, Universität Göttingen, Untere Karspüle 2, D-3400, Göttingen, Germany

    R. Tischner, H. Gleibs, S. Hillmer & D. G. Robinson

Authors
  1. R. Tischner
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  2. H. Gleibs
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  3. S. Hillmer
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  4. D. G. Robinson
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Tischner, R., Gleibs, H., Hillmer, S. et al. Physiological properties and cytological features of protoplasts prepared fromChlorella saccharophila . Protoplasma 139, 153–159 (1987). https://doi.org/10.1007/BF01282286

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  • DOI: https://doi.org/10.1007/BF01282286

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