Summary
ThePhysarum plasmodium shows rhythmic contractile activities with a period of a few min. Phases of the oscillation in the plasmodium migrating unindirectionally agreed sideways throughout at the frontal part. So, time course of an intracellular chemical component was determined by analyzing small pieces cut off successively from the frontal part of the large plasmodium. Intracellular NAD(P)H concentration oscillated with the same period as the rhythmic contraction but with a different phase advancing about 1/3 of the period. UV irradiation suppressed the rhythmic contraction without affecting the rhythmic variation of NAD(P)H. Thus, the NAD(P)H oscillator works independently of the rhythmic contractile system, but seems entraining with each other.
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Abbreviations
- UV:
-
ultraviolet
- NADH:
-
nicotinamide adenine dinucleotide, reduced form
- NADPH:
-
nicotinamide adenine dinucleotide phosphate, reduced form
- ATP:
-
adenosine 5′-triphosphate
- cAMP:
-
cyclic adenosine 3′, 5′-monophosphate
- FMNH2 :
-
flavin mononucleotide, reduced form
- TCA:
-
tricarboxylic acid
- BSA:
-
bovine serum albumin
- DTT:
-
dithiothreitol
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Mori, Y., Ueda, T. & Kobatake, Y. NAD(P)H oscillation in relation to the rhythmic contraction in thePhysarum plasmodium. Protoplasma 139, 141–144 (1987). https://doi.org/10.1007/BF01282284
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DOI: https://doi.org/10.1007/BF01282284