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Morphology and development of rhabdovirus-like particles inCuscuta odorata (Convolvulaceae) simultaneous infected with virus and mycoplasma-like organisms

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Summary

Electron microscopic examination ofCuscuta odorata, used for transmission trials, revealed mycoplasma-like organisms (MLO) as well as rhabdovirus-like particles, unknown toCuscuta. The virus infection is confined to certain phloem-parenchyma cells and a 1–2 cell thick layer of parenchyma cells with thickened walls surrounding the central cylinder. Virus particles, mostly bacilliform, could be detected mainly in the nucleus but also in the cytoplasm. They reach a length of 350–400 nm and a diameter of approximately 75 nm. Virus assembly takes place exclusively in the nucleus. Virus maturation occurs in membrane bound areas within the nucleus, which have no connection with the perinuclear space. Formation of nucleocapsids is always associated with a nuclear viroplasm. Envelopment of virus particles occurs in these membrane bound areas. Budding into the perinuclear space does not occur. Virus infection leads to degeneration and finally to death of the protoplast.

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Abbreviations

cy:

cytoplasm

m:

membrane stacks

mt:

mitochondria

my:

mycoplasma-like organisms

nc:

nucleocapsid

ncp:

nucleocapsid particles

nf:

nuclear filaments

np:

nucleoplasm

nu:

nucleus

nvp:

nuclear viroplasm

oc:

obliterated cells

p:

plastid

pc:

passage cells

ph:

phloem

ps:

perinuclear space

spc:

strand of parenchymatous cells

v:

virus particle

x:

xylem

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Authors and Affiliations

  1. Institute of Microbiology, Federal Biological Research Centre of Agriculture and Forestry, Berlin

    B. Kuhbandner, H. Petzold, R. Marwitz & W. Siller

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  1. B. Kuhbandner
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  2. H. Petzold
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  3. R. Marwitz
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  4. W. Siller
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Kuhbandner, B., Petzold, H., Marwitz, R. et al. Morphology and development of rhabdovirus-like particles inCuscuta odorata (Convolvulaceae) simultaneous infected with virus and mycoplasma-like organisms. Protoplasma 139, 130–140 (1987). https://doi.org/10.1007/BF01282283

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  • DOI: https://doi.org/10.1007/BF01282283

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