Summary
Changes in the abundance and form of endoplasmic reticulum in the three major cell types of the maize root cap were investigated by stereological and stereometric techniques. Quantification from thin sections was by the modification and application of standard morphometric procedures. This revealed dramatic increases in both the volume fraction and surface densities of endoplasmic reticulum as the meristem cells differentiate into starch and secretory cells. A stereometric technique for analysing thick sections was used to assess changes in the types of endoplasmic reticulum as cells differentiate through the root cap. This procedure showed that the proportions of cisternal endoplasmic reticulum to tubular endoplasmic reticulum was highest in the peripheral secretory cells. Electron opacity of the endomembrane system was enhanced by selective staining with zinc iodide and osmium tetroxide (ZIO).
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References
Barlow, P. W., 1970: Mitotic spindle and mitotic cell volume in the root mersitem ofZea mays. Planta91, 169–172.
—, 1974: Regeneration of the cap of primary roots ofZea mays L. New Phytol.73, 937–954.
—, 1978: Cell displacement through the columella of the root cap ofZea mays L. Ann. Bot.42, 783–790.
—,Hkwes, C. R., Horne, J. C., 1984: Structure of amyloplasts and endoplasmic reticulum in the root cap cells ofLepidium sativum andZea mays observed after selective membrane staining and by high-voltage electron microscopy. Planta160, 363–371.
—,Sargent, J. A., 1978: The ultrastructure of the regenerating root cap ofZea mays L. Ann. Bot.42, 791–799.
Bouck, G. B., 1963: Stratification and subsequent behaviour of plant organelles. J. Cell Biol.56, 340–359.
Chrispeels, M. J., 1980: The endoplasmic reticulum, pp. 389–412. In: The Biochemistry of Plants, Vol. 1 (Tolbert, N. E., ed.). New York: Academic Press.
Clowes, F. A. L., 1971: The proportion of cells that divide in root meristems ofZea mays L. Ann Bot.35, 249–261.
Dauwalder, M., Whaley, W. G., 1982: Membrane assembly and secretion in higher plants. J. Ultrastruct. Res.78, 302–320.
Dormer, K. J., 1980: Fundamental Geometry for Biologists. Cambridge, U.K.: Cambridge University Press.
Goldstein, S., Moerman, E. J., Porter, K., 1984: High-voltage electron microscopy of human diploid fibroblasts during ageingin vitro: Morphometric analysis of mitochondria. Exp. Cell Res.154, 101–111.
Green, J. R., Northcote, D. H., 1978: The structure and function of glycoproteins synthesized during slime-polysaccharide production by membranes of the root-cap cells of Maize (Zea mays). Biochem. J.170, 599–608.
Harris, N., 1978: Nuclear pore distribution and relation to adjacent cytoplasmic organelles of developingVicia faba. Planta141, 121–128.
—, 1979: Endoplasmic reticulum in developing seeds ofVicia faba. A high voltage electron microscope study. Planta146, 63–69.
—,Chrispeels, M. L., 1980: The endoplasmic reticulum of mung bean cotyledons: Quantitative morphology of cisternal and tubular ER during seedling growth. Planta148, 293–303.
—,Oparka, K. J., 1983: Connections between dictyosomes, ER and GERL in cytoledons of mung bean (Vigna radiata L.). Protoplasma114, 93–102.
Hawes, C. R., 1985: Conventional and high voltage electron microscopy of the cytoskeleton and cytoplasmic matrix of carrot (Daucus carota L.) cells grown in suspension culture. Eur. J. Cell Biol.38, 201–210.
—,Juniper, B. E., Horne, J. C., 1981: Low and high voltage microscopy of mitosis and cytokinesis in maize roots. Planta152, 397–407.
Hepler, P. K., 1982: Endoplasmic reticulum in the formation of the cell plate and plasmodesmata. Protoplasma111, 121–133.
Hensel, W., Sievers, A., 1980: Effects of prolonged omnilateral gravistimulation on the ultrastructure of statocytes and the graviresponse of roots. Planta150, 338–346.
Hucher, M., Grolier, J., 1977: Fifty-nine tetrakaidecahedra as grain models. J. Microscop.111, 329–337.
Jeffers, J. N. R., 1959: Experimental design and analysis in forest research. Stockholm:Almquist andWiksell.
Juniper, B. E., 1976: Geotropism. Ann. Rev. Plant Physiol.27, 385–406.
—,Clowes, F. A. L., 1965: Cytoplasmic organelles and cell growth in root caps. Nature208, 864–865.
—,French, A., 1973: The distribution and redistribution of endoplasmic reticulum (ER) in geoperceptive cells. Planta109, 211–224.
—,Hkwes, C. R., Horne, J. C., 1982: The relationship between the dictyosomes and the forms of the endoplasmic reticulum in plant cells with different export programs. Bot. Gaz.143, 135–145.
Mollenhauer, H. H., Mooré, D. J., 1976: Transition elements between Golgi apparatus in plant cells. Cytobiologie13, 297–306.
Morre, R., McCelen, C. E., 1983: A morphometric analysis of cellular differentiation in the root cap ofZea mays. Amer. J. Bot.70, 611–617.
— —, 1985: Changes in the distribution of plastids and endoplasmic reticulum during cellular differentiation in root caps ofZea mays. Ann Bot.56, 73–81.
Overall, R. L., Wolfe, J., Gunning, B. E. S., 1982: Intercellular communication inAzolla roots: 1. Ultrastructure of Plamodesmata. Protoplasma111, 134–150.
Ohno, S.,Yoshida, K.,Murata, F.,Nagata, T., 1978: Morphometry of cell organelles by high voltage electron microscopy. Electron microscopy (Sturgess, J. M., ed.)2, 14–15.
Parker, R. E., 1979: Introductory statistics for biology, 2nd edition. London: Arnold.
Paull, R. E., Johnston, G. M., Jones, R. L., 1975: Studies on the secretion of maize root cap slime. 1. Some properties of the secreted polymer. Plant Physiol.56, 300–306.
Quaite, E., Parker, R. E., Steer, M. W., 1983: Plant cell extension: structural implication for the origin of the plasma membrane. Plant, Cell and Environment6, 429–432.
Shannon, T. M., Steer, M. W., 1984: The root cap as a test system for the evaluation of Golgi inhibitors. 1. Structure and dynamics of the secretory system and response to solvents. J. exp. Bot.35, 1697–1707.
—,Henry, Y., Picton, J. M., Steer, M. W., 1982: Polarity in higher plant dictyosomes. Protoplasma112, 189–195.
Sievers, A., Heyder-Caspers, L., 1983: The effect of centrifugal accelerations on polarity of statocytes and on the graviperception of cress roots. Planta157, 61–70.
Steer, M. W., 1981: Understanding Cell Structure. Cambridge, U.K.: Cambridge University Press.
Snedecor, G. W., Cochran, W. G., 1980: Statistical Methods. (7th ed.) Ames, Iowa, U.S.A.: Iowa State University Press.
Spurr, A. R., 1969: A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res.26, 31–43.
Thomas, L. E.,Lentz, S.,Fisher, R. M., 1974: Stereoscopic methods in the HVEM. In: High Voltage Electron Microscopy, Proc. 3rd Int. Conf. (Swann, P. R.,Humphreys, C. J.,Goringe, M. J., eds.), pp. 255–259.
Weibel, E. R., 1979: Stereological methods. Vol. 1. Practical Methods for Biological Morphometry. London: Academic Press.
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Stephenson, J.L.M., Hawes, C.R. Stereology and stereometry of endoplasmic reticulum during differentiation in the maize root cap. Protoplasma 131, 32–46 (1986). https://doi.org/10.1007/BF01281685
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DOI: https://doi.org/10.1007/BF01281685