Summary
Microcuttings ofFraxinus excelsior, sampled from adult trees during the period of cell cycle blockage of bud in G0–1, developed long rejuvenated sprouts on the Woody Plant Medium (WPM) with benzylaminopurine (BAP) (4.0 mg/l) and indolebutyric acid (IBA) (0.03 mg/l). These sprouts had the ability to enter a resting period, building dormant-like buds when maintained on the original WPM. Sprouts developed from subcultures also entered a resting period without any transfer. Comparison of in nature buds in active growth and dormancy with buds of growing sprouts and in vitro dormant-like buds revealed similarity in behaviour at the shoot apical level. In particular, in dormant-like buds in a constant environment, shoot apical functioning was suppressed while the cell cycle of the shoot apex was blocked at the G0–1 phase, like in nature dormant buds.
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Abbreviations
- a.u.:
-
arbitrary unit
- BAP:
-
benzylaminopurine
- CF:
-
cumulative frequency
- d1, d2 :
-
diameters of the shoot apex
- IBA:
-
indolebutyric acid
- Pn :
-
last opposite primordia of range n
- WPM:
-
woody plant medium
References
Al Maarri K (1986) Micropropagation in vitro du Pommier (M9), du Poirier (“Williams” et “Passe crassane”) et du Cognassier de Provence: problèmes posés par l'état physiologique du matériel. Thèse de Doctorat, spécialité Sciences de la Vie. Biologie et Physiologie Végétales, Université Pierre et Marie Curie, Paris, France
Besnard-Wibaut C, Noin M, Zeevart JAD (1983) Mitotic activities and levels of nuclear DNA in the apical meristem ofSilene armeria (strain S1–2) following application of gibberellin A3. Plant Cell Physiol 24: 1269–1279
Boxus P (1971) La culture de méristèmes dePrunus. Note préliminaire relative à l'espèceP. pandora. Bull Rech Agron Gembloux 6: 3–5
— (1974) La culture de méristèmes dePrunus pour l'obtention de plants sains. Acta Hortic 44: 43–46
Brossard D (1973) Le bourgeonnement épiphylle chez leBryophyllum daigremontianum Berger, Crassulacées. Etude cytochimique, cytophotométrique et ultrastructurale. Ann Sci Nat Bot 12 Ser 14: 93–214
— (1975) La néoformation de bourgeons végétatifs à partir de la moelle de tabac (Nicotiana tabacum L. var. Wisconsin 38) cultivée in vitro. Analyse cytochimique, historadiographique et cytophotométriqiue. Ann Sci Nat Bot 12 Ser 16: 43–150
Brunori A, D'Amato F (1967) The DNA content of nuclei in the embryo of dry seeds ofPinus pinea andLactuca saliva. Caryologia 20: 154–161
Cottignies A (1979) The blockage in the G1 phase of the cell cycle in the dormant shoot apex of ash. Planta 147: 15–19
— (1985) Dormance et croissance active chez le Frêne (Fraxinus excelsior L.). Thése de Doctorat d'Etat, Université Pierre et Marie Curie, Paris, France
Greilhuber J (1986) Severely distorted Feulgen DNA amounts inPinus (Coniferophytina) after non additive fixation, as a result of meristematic self-tanning with vacuole contents. Can J Genet Cytol 28: 409–415
— (1988) Critical reassessment of DNA content variation in plants. In: Brandham PE (ed) Kew Chromosome Conference III. HMSO, London, pp 39–50
Guillaumin D (1980) La pratique du microscope électronique à balayage en biologie. Masson, Paris
Hosier MA, Flatebo G, Reade PE (1985) In vitro propagation in lingonberry. Hort Sci 20: 364–365
Huxley TJ, Cartwright PM (1994) Juvenility in micropropagated strawberries (Fragaria ananassa Duch). In: Lumsden PJ, Nicholas JR, Davies WJ (eds) Physiology, growth and development of plants in culture. Kluwer, Dordrecht, pp 346–355
Jemmali A (1994) Etude physiologique et morphogénétique de la floraison chez les microplants de fraisier (Fragaria Xananassa uch.) cv. Gorella. Thése de Doctorat, Faculté des Sciences Agronomiques de l'Etat, Gembloux, Belgium
Krul WR, Myerson J (1980) In vitro propagation of grape. In: Proceedings of the Conference on Nursery Production of Fruit Plants Through Tissue Culture. Applications and Feasibility. USDA-SEA Agricultural Research Results ARR-NE 11: 35–48
Lloyd G, McCown B (1981) Commercially feasible micropropagation of mountain laurel,Kalmia latifolia by use of shoot tip culture. Proc Int Plant Propag Soc 30: 421–427
Lyrene PM (1981) Juvenility and the production of fast-rooting cuttings from blueberry shoot cultures. J Am Soc Hort Sci 112: 125–130
Michaux N (1976) Etude comparative, histologique, histochimique et cytologique du bourgeon apical de I'Isoetes setacea durant les périodes de croissance active et de résistance à la sécheresse. Phytomorphology 26: 210–219
Nougarède A, Rembur J (1978) Variation of the cell cycle phases in the shoot apex ofChrysanthemum segetum. Z Pflanzenphysiol 90: 379–389
— — (1980) Extension cellulaire, division cellulaire et niveaux de ploidie des cellules corticales de l'épicotyle de pois en cours d'élongation. Can J Bot 58: 486–501
— — (1985) Le point végétatif en tant que modèle pour l'étude du cycle cellulaire et de ses points de contrôle. Bull Soc Bot Fr Actual Bot 132: 9–34
—, Rondet P (1975) Index mitotiques et teneurs en DNA nucléaire du méristème axillaire de la feuille de rang 6 et du point végétatif terminal, chez lePisum sativum L., var. nain hâtif d'Annonay. C R Acad Sci Sér D 280: 709–712
—, Tepfer SS, Tepfer M (1973) Les bourgeons au cours de l'état pérennant chey lePhytolacca decandra L. Modalités de conservation du plant durant l'hiver et reprise de l'activité printanière. C R Acad Sci Sér D 276: 957–960
—, Di Michele MN, Rondet P, Saint-Côme R (1990) Plastochrone, cycle cellulaire et teneurs en ADN nucléaire du méristème caulinaire de plants duChrysanthemum segetum soumis à deux conditions lumineuses différentes, sous une photopériode de 16 heures. Can J Bot 68: 2389–2397
—, Francis D, Rondet P (1991) Location of cell cycle changes in relation to morphological changes in the shoot apex ofSilene coeli-rosa immediately before sepal initiation. Protoplasma 165: 1–10
Patau K (1952) Absorption microphotometry of irregular-shaped objects. Chromosoma 5: 341–362
Powell LE (1988) The hormonal control of bud and seed dormancy in woody plants. In: Davies PJ (ed) Plant hormones and their role in plant growth and development. Martinus Nijhoff, Dordrecht, pp 539–552
Quoirin M, Lepoivre P, Boxus P (1977) Un premier bilan de 10 années de recherches sur les cultures de méristèmes et la multiplication in vitro de fruitiers ligneux. Comptes rendus des recherches 1976–1977. Station des Cultures Fruitières et Maraîchères, Gembloux
Rembur J, Nougarède A (1987) Microspectrophotometric measurements of DNA by automated computerised scanning in cycling cells of theChrysanthemum segetum shoot apex. Protoplasma 136: 183–190
Schart E, Landré P, Nougarède A (1985) Etat nucléaire des méristèmes du pois dans la graine sèche; imbibition et reprise du cycle cellulaire. Can J Bot 63: 2200–2208
Silveira CE (1994) Bouturage et microbouturage duFraxinus excelsior L: aspects morphogénétiques et cytophysiologiques. Thèse de Doctorat, Université Pierre et Marie Curie, Paris, France
—, Cottignies A (1994) Period of harvest, sprouting ability of cuttings and in vitro plant regeneration inFraxinus excelsior. Can J Bot 72: 261–267
—, Nougarède A (1995) Microbouturage deFraxinus excelsior L: la phase de multiplication, plaque tournante pour l'engagement dans diverses voies morphogénétiques. C R Acad Sci Sér III 313: 199–207
Tepfer SS, Nougarède A, Rondet P (1981) Seasonal studies of vegetative buds ofHelianthus tuberosus: concentration of nuclei in phase G1 during winter dormancy. Can J Bot 59: 1918–1927
Toupiol D (1976) Mise en place de l'inhibition du bourgeon cotylédonaire chez lePisum sativum L. Etude morphologique et cytophotométrique. C R Acad Sci Sér D 282: 281–284
Wareing PF (1987) Juvenility and cell determination. In: Atherton JB (ed) Manipulation of flowering. Butterworths, London, pp 83–92
—, Phillips IDJ (1986) Growth and differentiation in plants, 3rd edn. Pergamon, Oxford
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Nougarède, A., Silveira, C.E. & Rondet, P. In nature dormant buds and in vitro dormant-like buds ofFraxinus excelsior L.. Protoplasma 190, 16–24 (1996). https://doi.org/10.1007/BF01281191
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DOI: https://doi.org/10.1007/BF01281191