Skip to main content
SpringerLink
Log in

Characterization and chromosomal localization of fast renaturing and satellite DNA sequences inPhaseolus coccineus

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

DNA was extracted from etiolated plantlets ofPhaseolus coccineus L. and studied by means of thermal denaturation and reassociation kinetics and CsCl analytical ultracentrifugation. A DNA fraction reassociating within an equivalent Cot of 1.15 × 10−1 (fast renaturing DNA sequences; FRS) was found to constitute 20.8% of the total DNA, 6.8% of which is composed of palindromic sequences. Analytical CsCl gradient ultracentrifugation showed a large heavier satellite banding at 1.702 g/ml (main peak 1.692g/ml); this DNA fraction was shown to consist of different components with buoyant densities of 1.696, 1.702, 1.706, and 1.714g/ml. FRS and the satellite fraction banding at 1.702 g/ml (SAT) were hybridizedin situ to the polytene chromosomes in the embryo suspensor cells after having been labeled by the nick translation method. FRS proved to be almost exclusively localized in the heterochromatic chromosome regions and several chromosome ends; SAT was found to be confined, as a rule, in the centromeric and pericentromeric heterochromatin. Different labeling patterns were detected over chromosome regions bearing 18S + 25S or 5 S ribosomal RNA cistrons and over corresponding regions of different chromosome pairs. These results are discussed for the light they may throw on certain aspects of the structure and organization at the chromosomal level of theP. coccineus genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alpi A, Tognoni F, D'Amato F(1975) Growth regulator levels in embryo and suspensor ofPhaseolus coccineus at two stages of development. Planta 127: 153–162

    Google Scholar 

  • Appels R, Dennis ES, Smyth DR, Peacock WJ (1981) Two repeated DNA sequences from the heterochromatic regions of rye (Secale cereale) chromosomes. Chromosoma 84: 265–277

    Google Scholar 

  • —,Driscoll C, Peacock WJ (1978) Heterochromatin and highly repeated DNA sequences in rye (Secale cereale). Chromosoma 70: 67–89

    Google Scholar 

  • Avanzi S, Cionini PG, D'Amato F (1970) Cytochemical and autoradiographic analyses on the embryo suspensor cells ofPhaseolus coccineus. Caryologia 23: 605–638

    Google Scholar 

  • —,Durante M, Cionini PG, D'Amato F (1972) Cytological localization of ribosomal cistrons in polytene chromosomes ofPhaseolus coccineus. Chromosoma 39: 191–203

    Google Scholar 

  • Bassi P, Cremonini R, Cionini PG (1982) Cytological localization of inverted repeated DNA sequences inVicia faba. Chromosoma 85: 453–459

    Google Scholar 

  • Beridze T (1980) Properties of satellite DNAs of higher plants. Plant Sci Lett 19: 325–338

    Google Scholar 

  • Brady T (1973) Feulgen cytophotometric determination of the DNA content of the embryo proper and suspensor cells ofPhaseolus coccineus. Cell Differ 2: 65–75

    Google Scholar 

  • Britten RJ, Graham DE, Neufeld BR (1974) Analysis of repeated DNA sequences by reassociation. Meth Enzymol 29: 363–405

    Google Scholar 

  • Cionini PG (1975) Pattern of binding of tritiated actinomycin D toPhaseolus coccineus polytene chromosomes. II. The entire chromosome complement. Giorn Bot Ital 109: 183–192

    Google Scholar 

  • —,Avanzi S (1972) Pattern of binding of tritiated actinomycin D toPhaseolus coccineus polytene chromosomes. I. Nucleolus organizing chromosomes. Exp Cell Res 75: 154–158

    Google Scholar 

  • —,Bennici A, Alpi A, D'Amato F (1976) Suspensor, gibberellin andin vitro development ofPhaseolus coccineus embryos. Planta 131: 115–117

    Google Scholar 

  • —,Cavallini A, Corsi R, Fogli M (1982) Comparison of homologous polytene chromosomes inPhaseolus coccineus embryo suspensor cells: morphological, autoradiographic and cytophotometric analyses. Chromosoma 86: 383–396

    Google Scholar 

  • Cionini PG, Cremonini R, Bassi P (1984) Highly repeated DNA sequences and the structural organization of plant cell nuclei. Ann Bot (Roma) 42: 29–44

    Google Scholar 

  • Comings DE, Avelino E, Okada TA, Wyandt HE (1973) The mechanism of C- and G-banding of chromosomes. Exptl Cell Res 77: 469–493

    Google Scholar 

  • Cremonini R, Cionini PG (1977) Extra DNA synthesis in embryo suspensor cells ofPhaseolus coccineus. Protoplasma 99: 303–313

    Google Scholar 

  • Durante M (1979) Molecular biology ofPhaseolus suspensor. In: The plant genome. Proc. IV John Innes Symp., 47–60. The John Innes Charity, Norwich

    Google Scholar 

  • —,Cionini PG, Avanzi S, Cremonini R, D'Amato F (1977 a) Cytological localization of the genes for the four classes of ribosomal RNA (25 S, 18 S, 5.8 S, and 5 S) in polytene chromosomes ofPhaseolus coccineus. Chromosoma 60: 269–282

    Google Scholar 

  • —,Cremonini R, Brunori A, Avanzi S, Innocenti AM (1977 b) Differentiation of metaxylem cell line in the root ofAllium cepa. I. DNA heterogeneity and ribosomal cistrons of two different stages of differentiation. Protoplasma 93: 289–303

    Google Scholar 

  • Forino LMC, Cremonini R, Tagliasacchi AM (1976) Frequenza di cellule con “DNA puffs” nel sospensore diPhaseolus coccineus in momenti diversi dello sviluppo embrionale. Giorn Bot Ital 110: 337–346

    Google Scholar 

  • —,Tagliasacchi AM, Avanzi S (1979) Different structure of polytene chromosomes ofPhaseolus coccineus suspensors during early embryogenesis. 1. Nucleolus organizing chromosome pairs S1 and S2. Protoplasma 101: 231–246

    Google Scholar 

  • Frediani M, Forino LMC, Tagliasacchi AM, Cionini PG, Durante M, Avanzi S (1986 a) Functional heterogeneity, during early embryogenesis, ofPhaseolus coccineus ribosomal cistrons in polytene chromosomes of embryo suspensor. Protoplasma 132: 51–57

    Google Scholar 

  • —,Tagliasacchi AM, Durante M, Avanzi S (1986 b) Distribution of 5-methylcytosine-rich regions in the polytene chromosomes ofPhaseolus coccineus embryo suspensor as shown by the immunoperoxidase technique. Exptl Cell Res 167: 337–342

    Google Scholar 

  • Greilhuber J (1975) Heterogeneity of heterochromatin in plants: comparison of Hy- and C-bands inVicia faba. Plant Syst Evol 124: 139–156

    Google Scholar 

  • Hennen S, Mizuno S, Macgregor HC (1975)In situ hybridization of ribosomal DNA labeled with125iodine to metaphase and lampbrush chromosomes from newts. Chromosoma 50: 349–369

    Google Scholar 

  • Lima-de-Faria A (1983) Molecular evolution and organization of the chromosome. Elsevier, Amsterdam New York Oxford

    Google Scholar 

  • —,Pero R, Avanzi S, Durante M, StÅhle U, D'Amato F, Granström H (1975) Relation between ribosomal RNA genes and the DNA satellites ofPhaseolus coccineus. Hereditas 79: 5–20

    Google Scholar 

  • Lorenzi R, Bennici A, Cionini PG, Alpi A, D'Amato F (1978) Embryo-suspensor relations inPhaseolus coccineus: cytokinins during seed development. Planta 143: 59–62

    Google Scholar 

  • Macgregor HC, Mizuno S (1976)In situ hybridization of “nick translated”3H-ribosomal DNA to chromosomes of salamanders. Chromosoma 54: 15–25

    Google Scholar 

  • Marsh JL, McCarty BJ (1974) Effect of reaction conditions on the reassociation of divergent deoxyribonucleic acid sequences. Biochemistry 13: 3382–3388

    Google Scholar 

  • Miklos GLG, Nankivell RN (1976) Telomeric satellite DNA functions in regulating recombination. Chromosoma 56: 143–167

    Google Scholar 

  • Nagl W (1967) Die Riesenchromosomen vonPhaseolus coccineus L.: Baueigentümlichkeiten, Strukturmodifikationen, zusätzliche Nukleolen und Vergleich mit dem mitotischen Chromosomen. Öst Bot Z 114: 171–182

    Google Scholar 

  • —, (1970) Temperature-dependent functional structures in the polytene chromosomes ofPhaseolus, with special reference to the nucleolus organizers. J Cell Sci 6: 87–107

    Google Scholar 

  • —, (1974) ThePhaseolus suspensor and its polytene chromosomes. Z Pflanzenphysiol 73: 1–44

    Google Scholar 

  • Renkawitz-Pohl R, Bialojan S (1984) A DNA sequence ofDrosophila melanogaster with a differential telomeric distribution. Chromosoma 89: 206–211

    Google Scholar 

  • Rigby WJ, Dieckmann M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activityin vitro by nicktranslation with DNA polymerase I. J Mol Biol 113: 237–251

    Google Scholar 

  • Rowland RE (1981) Chromosomal banding and heterochromatin inVicia faba. Theor Appl Genet 60: 275–280

    Google Scholar 

  • Schildkraut CL, Lifson S (1965) Dependence of the melting temperature of DNA on salt concentration. Biopolymers 3: 195–208

    Google Scholar 

  • —,Marmur J, Doty P (1962) Determination of the base composition of the deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol 4: 430–443

    Google Scholar 

  • Tagliasacchi AM, Forino LMC, Cionini PG, Cavallini A, Durante M, Cremonini R, Avanzi S (1984) Different structure of polytene chromosomes ofPhaseolus coccineus suspensors during early embryogenesis. 3. Chromosome pair VI. Protoplasma 122: 98–107

    Google Scholar 

  • —,Forino LMC, Frediani M, Avanzi S (1983) Different structure of polytene chromosomes ofPhaseolus coccineus suspensors during early embryogenesis. 2. Chromosome pair VII. Protoplasma 115: 95–103

    Google Scholar 

  • Viegas-Pequignot E, Benazzou T, Prod'homme M, Dutrillaux B (1984) Characterization of a very complex constitutive heterochromatin in twoGerbillus species (Rodentia). Chromosoma 89: 42–47

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Genetica della Università, Pisa

    M. Durante

  2. Dipartimento di Scienze Botaniche della Università, via L. Ghini 5, I-56100, Pisa, Italy

    R. Cremonini, A. M. Tagliasacchi, L. M. C. Forino & P. G. Cionini

Authors
  1. M. Durante
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Cremonini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. M. Tagliasacchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. M. C. Forino
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. G. Cionini
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Durante, M., Cremonini, R., Tagliasacchi, A.M. et al. Characterization and chromosomal localization of fast renaturing and satellite DNA sequences inPhaseolus coccineus . Protoplasma 137, 100–108 (1987). https://doi.org/10.1007/BF01281145

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01281145

Keywords

Search

Navigation