Summary
Isolated mesophyll cells ofZinnia elegans L. cv. Canary Bird differentiate into tracheary elements in differentiation (D) medium. These elements develop lignified secondary wall thickenings. The influence of 2-aminoindan-2-phosphonic acid (AIP), an inhibitor of phenylalanine ammonia-lyase (PAL), on lignification ofZinnia tracheary elements was examined. The mesophyll cells were cultured in D and AIP media. The latter medium, in which 100 μM AIP was added to the D medium, inhibited PAL activity, though the differentiation proceeded. Morphological differences of secondary wall thickenings cultured in these two types of media were investigated under an UV microscope and a transmission electron microscope. The secondary wall thickenings at 96 h in the D medium showed strong UV absorption. The fibrillar structure of the thickenings observed clearly at 72 h was covered with electron opaque materials by 96 h. The secondary wall thickenings at 96 h in the AIP medium showed weak UV absorption. The thickenings at 96 h had a cracked appearance. Furthermore, the thickenings showed a little irregular or wavy arrangement of cellulose microfibrils and had many pores and spaces between microfibrils. From these results, the role of lignin accumulation in the formation of secondary wall thickenings was discussed.
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Abbreviations
- AIP:
-
2-aminoindan-2-phosphonic acid
- PAL:
-
phenylalanine ammonia-lyase
References
Amrhein N, Frank G, Lemm G, Luhmann HB (1983) Inhibition of lignin formation by L-α-aminooxy-β-phenylpropionic acid — an inhibitor of phenylalanine ammonia-lyase. Eur J Cell Biol 29: 139–144
Brown RM (ed) (1982) Cellulose and other natural polymer systems: biogenesis, structure and degradation. Plenum, New York
Fukuda H, Komamine A (1980) Establishment of an experimental system for the study of tracheary element differentiation from single cells isolated from the mesophyll ofZinnia elegans. Plant Physiol 65: 57–60
— — (1982) Lignin synthesis and its related enzymes as markers of tracheary-element differentiation in single cells isolated from the mesophyll ofZinnia elegans. Planta 155: 67–70
Haddon L, Northcote DH (1975) Quantitative measurement of the course of bean callus differentiation. J Cell Sci 7: 11–26
Ingold I, Sugiyama M, Komamine A (1990) L-α-aminooxy-β- phenylpropionic acid inhibits lignification but not the differentiation to tracheary elements of isolated mesophyll cells ofZinnia elegans. Physiol Plant 78: 67–74
Rubery PH, Fosket DE (1969) Changes in phenylalanine ammonialyase activity during xylem differentiation inColeus and soybean. Planta 87: 54–62
—, Northcote DH (1968) Site of phenylalanine ammonia-lyase activity and synthesis of lignin during xylem differentiation. Nature 219: 1230–1234
Sato Y, Sugiyama M, Gorecki RJ, Fukuda H, Kamamine A (1993) Interrelationship between lignin deposition and the activities of peroxidase isoenzymes in differentiating tracheary elements ofZinnia. Planta 189: 584–589
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Nakashima, J., Takabe, K., Fujita, M. et al. Inhibition of phenylalanine ammonia-lyase activity causes the depression of lignin accumulation of secondary wall thickening in isolatedZinnia mesophyll cells. Protoplasma 196, 99–107 (1997). https://doi.org/10.1007/BF01281063
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DOI: https://doi.org/10.1007/BF01281063