Summary
In the cells of the fungiNeurospora crassa, Rhizopus nigricans, andSaccharomyces cerevisiae catalase was detected in distinct granula with the cytochemical procedure ofFahimi (1968). The specificity of the staining reaction was demonstrated by the inhibitory action of 3-amino-1,2,4-triazole.
Zusammenfassung
Mit Hilfe der cytochemischen Methode vonFahimi (1968) wurde in den Zellen der PilzeNeurospora crassa, Rhizopus nigricans undSaccbaromyces cerevisiae das Enzym Katalase in distinkten Granula nachgewiesen. Die Spezifität der Färbereaktion wurde durch ihre Hemmbarkeit mit 3-Amino-1,2,4-triazol gezeigt.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
Avers, C. J., andM. Federman, 1968: The occurrence in yeast of cytoplasmic granules which resemble microbodies. J. Cell Biol.37, 555–559.
Cochrane, V. W., 1963: Physiology of Fungi. New York and London: J. Wiley & Sons.
De Duve, C., andP. Baudhuin, 1966: Peroxisomes (microbodies and related particles). Physiol. Rev.46, 323–357.
Fahimi, H. D., 1968: Cytochemical localization of peroxidase activity in rat hepatic microbodies (peroxisomes). J. Histochem. Cytochem.16, 547–550.
—, 1969: Cytochemical localization of peroxidatic activity of catalase in rat hepatic microbodies (peroxisomes). J. Cell Biol.43, 275–288.
Frederick, S. E., andE. H. Newcomb, 1969: Cytochemical localization of catalase in leaf microbodies (peroxisomes). J. Cell Biol.43, 343–353.
Goldfischer, S., 1967: The cytochemical localization of myoglobin in striated muscle of man and walrus. J. Cell Biol.34, 398.
Graham, R. C. Jr., andM. J. Karnovsky, 1966: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem.14, 291–302.
Hruban, Z., and M.Rechcigl, Jr., 1969: Microbodies and related particles. Morphology, biochemistry, and physiology. Int. Rev. Cytol., Suppl. 1.
Karnovsky, M. J., 1965: Vesicular transport of exogenous peroxidase across capillary endothelium into the T-system of muscle. J. Cell Biol.27, 49 A.
Margoliash, E., andA. Novogrodsky, 1958: A study of the inhibition of catalase by 3-amino-1/3n2∶∶4-triazole. Biochem. J.68, 468–475.
Mollenhauer, H. H., D. J. Morré, andA. G. Kelley, 1966: The widespread occurrence of plant cytosomes resembling animal microbodies. Protoplasma62, 44–52.
Novikoff, A. B., andS. Goldfischer, 1968: Visualization of microbodies for light and electron microscopy. J. Histochem. Cytochem.16, 507.
Perlman, P. S., andH. R. Mahler, 1970: Intracellular localization of enzymes in yeast. Arch. Biochem. Biophys.136, 245–259.
Pitt, D., 1969: Cytochemical evidence for the existence of peroxisomes inBotrytis cinerea. J. Histochem. Cytochem.17, 613–616.
Reiss, J., 1967: Der cytochemische Nachweis von Dehydrogenasen bei Pilzen. I. Untersuchungen und Ausblicke zur Methodik. Arch. Mikrobiol.57, 285–306.
Seligman, A. M., M. J. Karnovsky, H. L. Wasserkrug, andJ. S. Hanker, 1968: Nondroplet ultrastructural demonstration of cytochrome oxidase activity with a polymerizing osmiophilic reagent, diaminobenzidine (DAB). J. Cell Biol.38, 1–4.
Tolbert, N. E., A. Oeser, R. K. Yamazaki, R. H. Hageman, andT. Kisaki, 1969: A survey of plants for leaf peroxisomes. Plant Physiol.44, 135–147.
Vigil, E. L., 1969: Intracellular localization of catalase (peroxidatic) activity in plant microbodies. J. Histochem. Cytochem.17, 425–428.
Author information
Authors and Affiliations
Additional information
Frau Prof. Dr. B.Haccius danke ich für die Überlassung der Pilzkulturen.
Rights and permissions
About this article
Cite this article
Reiss, J. Cytochemische Darstellung von Peroxisomen in Pilzzellen. Protoplasma 72, 43–48 (1971). https://doi.org/10.1007/BF01281009
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01281009