Summary
The effect of colchicine upon the embryos of barley (Hordeum vulgare L.) culturedin vitro has been studied. Different concentrations of the drug as well as different times of culture in its presence have been tested, in order to ascertain the optimal conditions for the induction of non diploid cells. Tetraploid cells were always infrequent after the treatments, reaching the higher values during the first days of culture; aneuploid cells being frequent in some cases.
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References
Bayliss, M. W., 1976: Variation of cell cycle duration within suspension cultures ofDaucus carota and its consequence for the induction of ploidy changes with colchicine. Protoplasma88, 279–285.
Chen, C. H., Goeden-Kallemeyn, Y. C., 1979:In vitro induction of tetraploid plants from colchicine-treated diploid daylily callus. Euphytica28, 705–709.
Cheng, T. Y., Smith, H. H., 1975: Organogenesis from callus culture ofHordeum vulgare L. Planta123, 307–310.
D'amato, F., 1977: Cytogenetics of differentiation in tissue and cell cultures. In: Plant cell, tissue and organ culture (Reinert, J., Bajaj, Y. P. S., eds.), pp. 343–357. Berlin-Heidelberg-New York: Springer.
Espino, F. J., Vázquez, A. M., 1981: Chromosome numbers ofSaintpaulia ionanta plantlets regenerated from leaves culturedin vitro with caffeine and colchicine. Euphytica30, 847–854.
Heinz, D. J., Mee, G. W. P., 1970: Colchicine-induced polyploids from cell suspension cultures of sugarcane. Crop Science10, 696–699.
— —,Nickell, L. G., 1969: Chromosome numbers of someSaccharum species hybrids and their cell suspension cultures. Amer. J. Bot.56, 450–456.
Liu, M. C., Shang, K. C., Chen, W. H., Shih, S. C., 1977: Tissue and cell culture as aids to sugarcane breeding. III. Aneuploid cells and plants induced by treatment of cell suspension cultures with colchicine. Proc. Congr. Int. Soc. Sugar Cane Technol. (Brazil)16, 1–13.
Murashige, T., Nakano, R., 1966: Tissue culture as a potential tool in obtaining polyploid plants. J. Hered.57, 114–118.
Ruiz, M. L., Vázquez, A. M., 1981: Cell population evolution in tissue cultures from embryo barley (Hordeum vulgare L.) after caffeine treatment. Protoplasma107, 13–20.
Sacristan, M. D., Lutz, A. M., 1970: Etude du nombre chromosomique sur des plantes de tabac d'origine unicellulaire. C. R. Acad. Cs.270, 1334–1336.
Shimada, T., Sasakuma, T., Tsunewaki, K., 1969:In vitro culture wheat tissues. I. Callus formation, organ differentiation and single cell culture. Can. J. Genet. Cytol.11, 294–304.
Webster, P. L., Davidson, D., 1969: Changes in the duration of the mitotic cycle induced by colchicine and indol-3yl-acetic acid inVicia faba roots. J. exp. Bot.20, 671–685.
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Ruiz, M.L., Vazquez, A.M. Colchicine effect on the chromosome number of barley embryos culturedin vitro . Protoplasma 113, 237–240 (1982). https://doi.org/10.1007/BF01280913
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DOI: https://doi.org/10.1007/BF01280913