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γ-Tubulin is a component of the Spitzenkörper and centrosomes in hyphal-tip cells of Allomyces macrogynus

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Summary

A monoclonal antibody was used to localize γ-tubulin in hyphal tip cells of the chytridiomycete fungus Allomyces macrogynus, and its distribution determined with standard epifluorescence and laser scanning confocal microscopy. The results demonstrate that γ-tubulin is a component of the Spitzenkörper and centrosomes. Immunoblot analysis of total soluble protein extracts separated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis identified a single 56 kDa γ-tubulin-related polypeptide. Localization of γ-tubulin to the Spitzenkörper of A. macrogynus provides evidence that the Spitzenkörper in this fungus functions as a microtubule-organizing center.

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Abbreviations

BSA:

bovine serum albumin

DAPI:

4′,6-diamidino-2-phenylindole

DIC:

differential interference contrast

LSCM:

laser scanning confocal microscopy

MTOCs:

microtubule-organizing centers

PBS:

phosphate-buffered saline

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

SPB:

spindle pole body

YpSs:

yeast extract-inorganic phosphate-soluble starch

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Authors and Affiliations

  1. Department of Plant Biology, Arizona State University, Box 871601, 85287-1601, Tempe, AZ, USA

    D. P. McDaniel & R. W. Roberson

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  1. D. P. McDaniel
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  2. R. W. Roberson
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McDaniel, D.P., Roberson, R.W. γ-Tubulin is a component of the Spitzenkörper and centrosomes in hyphal-tip cells of Allomyces macrogynus . Protoplasma 203, 118–123 (1998). https://doi.org/10.1007/BF01280594

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