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In vivo studies on the nuclear behavior of the arbuscular mycorrhizal fungus Gigaspora rosea grown under axenic conditions

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Summary

The distribution and fate of nuclei of the arbuscular-my-corrhizal fungus Gigaspora rosea during late stages of axenic cultures were studied in fixed cultures by transmitted light, conventional and confocal laser scanning microscopy, and in live cultures with two-photon fluorescence microscopy. Mature specimens not yet showing apical septation displayed oval-shaped nuclei localized in lateral positions of the hypha all along the germ-tube length. Beside these, round-shaped nuclei were found to migrate along the central germ-tube core. Some (rare) germ-tube areas, delimited by septa and containing irregularly shaped, much brighter fluorescent nuclei were also found. Specimens that had just initiated the septation process after germ-tube growth arrest displayed round or oval-shaped nuclei in several portions of the germ tubes. These hyphal areas often alternated with other septa-delimited cytoplasmic clusters which contained distorted, brightly fluorescent nuclei. Completely septated specimens mostly lacked nuclei along their germ tubes. However, highly fluorescent chromatin masses appeared within remnants of cytoplasmic material, often compressed between close septa. Our results provide a first clear picture of the in vivo distribution of nuclei along arbuscular mycorrhizal fungal germ tubes issued from resting spores, and suggest that selective areas of their coenocytic hyphae are under specific, single nuclear control. They indicate as well that random autolytic processes occur along senescing G. rosea germ tubes, probably as a consequence of the absence of a host root signal for mycorrhizal formation. Finally, the data presented here allow us to envisage the fate of nuclei released by the germinating spore after nonsymbiotic fungal growth arrest.

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Abbreviations

AM:

fungi arbuscular-mycorrhizal fungi

DAPI:

4′, 6-diamidino-2-phenylindole

FM:

fluorescence microscopy

CLSM:

confocal laser scanning microscopy

2PM:

two-photon microscopy

PI:

propidium iodide

PMT:

photomultiplier tube

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  1. B. Bago

    Present address: Eastern Regional Research Center, ARS, U.S. Department of Agriculture, Plant and Soil Biophysics Laboratory, 600 E. Mermaid Lane, 19038, Wyndmoor, PA, USA

Authors and Affiliations

  1. Centre de Recherche en Biologie Forestière, Université Laval, Ste-Foy, Québec

    B. Bago & Y. Piché

  2. Applied and Engineering Physics, Cornell University, Ithaca, New York

    W. Zipfel, R. M. Williams & W. W. Webb

  3. Département de Biologie, Université Laval, Ste-Foy, Québec

    H. Chamberland & J. G. Lafontaine

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  1. B. Bago
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Bago, B., Zipfel, W., Williams, R.M. et al. In vivo studies on the nuclear behavior of the arbuscular mycorrhizal fungus Gigaspora rosea grown under axenic conditions. Protoplasma 203, 1–15 (1998). https://doi.org/10.1007/BF01280582

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