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Cytological abnormalities during culture ofNigella sativa

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Summary

Callus cultures were obtained from seeds ofNigella sativa L. on a modified basal medium ofMurashige andSkoog (1962). Cytological studies were carried out from the tissues during different periods of growth. Various cytological abnormalities like high and low chromosome number, stickiness, fragmentation, sticky bridges, lagging of chromosomes and multipolarity were observed in all the passages. The way of elimination of extra chromatin material after long cultures has been pointed out.

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Authors and Affiliations

  1. Centre of Advanced Study for Cell and Chromosome Research, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, 700 019, Calcutta, India

    Suresh Chand & Satyesh Chandra Roy

Authors
  1. Suresh Chand
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  2. Satyesh Chandra Roy
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Chand, S., Roy, S.C. Cytological abnormalities during culture ofNigella sativa . Protoplasma 104, 353–357 (1980). https://doi.org/10.1007/BF01279778

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  • DOI: https://doi.org/10.1007/BF01279778

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