Summary
The effect of low temperature (2 °C) on cell shape and microtubules in zoospores of the green algaChlorosarcinopsis gelatinosa has been investigated. The zoospores are 4–6 times longer than wide with a mean length of 12,5 μm and can be kept in the dark for several hours without changes in cell shape. Cell shape changes have been evaluated quantitatively by measuring changes in cell length. Low temperature induces a decrease in cell length which exhibits a two-step kinetic: during the first 30 minutes a rapid rate of decrease in cell length was measured, while during the next 4 hours a slow rate of decrease in cell length was observed. Complete regeneration of zoospore length occurs when cold-treated cells are subjected to the original zoospore induction temperature (30 °C) for two hours. Observation of numbers, disposition and types of microtubules in the zoospore during decrease in cell length has shown that within 30 minutes after cold application the secondary cytoskeletal microtubules (scmt) disappear, while flagellar root microtubules are unaffected. During this period most cells develop a prominent posterior appendage (tail). Sections demonstrate the presence of several microtubules in these tails. Flagellar root microtubules probably extend into the tails and disappearance of scmt starts at the posterior pole of the cell. Regeneration of zoospores to original cell length is coupled with reappearance of scmt starting at the anterior pole of the cell. It is concluded that secondary cytoskeletal microtubules constitute the main cytoskeleton inChlorosarcinopsis zoospores and that flagellar root microtubules contribute to only a minor extent to the cytoskeleton, because they cannot retain the cell shape. The results are discussed with respect to the functional significance of flagellar root microtubules in green algae.
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References
Borisy, G. G., 1978: Polarity of microtubules of the mitotic spindle. J. mol. Biol.124, 565–570.
Brown, R. M.,jr., Bold, H. C., 1964: Phycological studies. V. Comparative studies of the algal generaTetracystis andChlorococcum. Univ. of Texas Publ. No. 6417, Austin, Texas.
Goniakowska-Witalinska, L., Witalinski, W., 1976: Evidence for a correlation between the number of marginal band microtubules and the size of vertebrate erythrocytes. J. Cell Sci.22, 397–401.
Goode, D., 1973: Kinetics of microtubule formation after cold disaggregation of the mitotic apparatus. J. mol. Biol.80, 531–538.
Groover, R. D.,Bold, H. C., 1969: Phycological studies. VIII. The taxonomy and comparative physiology of theChlorosarcinales and certain other edaphic algae. Univ. of Texas Publ. No. 6907, Austin, Texas.
Hardham, A. R., Gunning, B. E. S., 1979: Interpolation of microtubules into cortical arrays during cell elongation and differentiation in roots ofAzolla pinnata. J. Cell Sci.37, 411–442.
Kessler, E., Czygan, F.-C., 1970: Physiologische und biochemische Beiträge zur Taxonomie der GattungChlorella. IV. Verwertung organischer Stickstoffverbindungen. Arch. Mikrobiol.70, 211–216.
Lembi, C. A., 1975: The fine structure of the flagellar apparatus ofCarteria. J. Phycol.11, 1–9.
Marchant, H. J., Pickett-Heaps, J. D., 1974: The effect of colchicine on colony formation in the algaeHydrodictyon, Pediastrum andSorastrum. Planta116, 291–300.
Melkonian, M., 1975: The fine structure of the zoospores ofFritschiella tuberosa Iyeng. (Chaetophorineae, Chlorophyceae) with special reference to the flagellar apparatus. Protoplasma86, 391–404.
—, 1977: The flagellar root system of zoospores of the green algaChlorosarcinopsis (Chlorosarcinales) as compared withChlamydomonas (Volvocales). Plant Syst. Evol.128, 79–88.
—, 1978: Structure and significance of cruciate flagellar root system in green algae: comparative investigations in species ofChlorosarcinopsis (Chlorosarcinales). Plant Syst. Evol.130, 265–292.
- 1980: Flagellar roots, mating structure and gametic fusion in the green algaUlva lactuca (Ulvales). J. Cell Sci. (in press).
-Robenek, H., 1980: Eyespot membranes ofChlamydomonas reinhardii: A freeze-fracture study. J. Ultrastruct. Res. (in press).
Moestrup, Ø., 1978: On the phylogenetic validity of the flagellar apparatus in green algae and other chlorophyll a and b containing plants. BioSystems10, 117–144.
Pickett-Heaps, J. D., 1975 a: Green algae. Structure, reproduction and evolution in selected genera. Sunderland, Mass.: Sinauer Assoc.
—, 1975 b: Structural and phylogenetic aspects of microtubular systems in gametes and zoospores of certain green algae. In: The biology of the male gamete (Duckett, J. G., Racey, P. A., eds.), pp. 37–44. London: Academic Press.
Raff, E. C., 1979: The control of microtubule assemblyin vivo. Intern. Rev. Cytol.59, 2–96.
Ringo, D. L., 1967: Flagellar motion and fine structure of the flagellar apparatus inChlamydomonas. J. Cell Biol.33, 543–571.
Roberts, K., 1974: Cytoplasmic microtubules and their functions. Progr. Biophys. Mol. Biol.28, 371–420.
Satir, P.,Ojakian, G. K., 1979: Plant cilia. In: Physiology of movements (Encyclopedia of plant physiology) Vol. 7 (Haupt, W.,Feinleib, M. E., eds.), pp. 224–249.
Smith, R. L., Wiedeman, V. E., 1964: A new alkaline growth medium for algae. Can. J. Bot.42, 1582–1586.
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Melkonian, M., Kröger, K.H. & Marquardt, K.G. Cell shape and microtubules in zoospores of the green algaChlorosarcinopsis gelatinosa (Chlorosarcinales): Effects of low temperature. Protoplasma 104, 283–293 (1980). https://doi.org/10.1007/BF01279773
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DOI: https://doi.org/10.1007/BF01279773