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Protoplasma

, Volume 124, Issue 1–2, pp 80–86 | Cite as

Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood (Santalum album L.)

  • P. S. Rao
  • Peggy Ozias-Akins
Article

Summary

Protoplasts were isolated from embryogenic cell suspension cultures derived from proliferating shoot segments of a 20-year-old sandalwood tree (Santalum albumLinn.). Under appropriate conditions, isolated protoplasts divided in liquid culture medium and produced embryogenic cell aggregates and globular embryos. Plating of cell aggregates on a fresh medium facilitated the differentiation of somatic emryos which further developed into plantlets.

Keywords

Santalum album L. Sandalwood Somatic embryogenesis tree protoplasts 

Abbreviations

BAP

6-benzylaminopurine

2,4-D

2,4-dichlorophenoxyacetic acid

GA3

Gibberellic acid

IAA

indoleacetic acid

IBA

indolebutrytic acid

MES

2-(N-morpholino)ethane sulfonic acid

MS

Murashige and Skoog's medium as modified in the text

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References

  1. Backs-Hüsemann, D., Reinert, J., 1970: Embryobildung durch isolierte Einzelzellen aus Gewebekulturen vonDaucus carota. Protoplasma70, 49–60.Google Scholar
  2. Bapat, V. A., Rao, P. S., 1979: Somatic embryogenesis and plantlet formation in tissue cultures of sandalwood (Santalum album L.). Ann. Bot.44, 629–630.Google Scholar
  3. — —, 1984: Regulatory factors forin vitro multiplication of sandalwood tree (Santalum album L.) I. Shoot bud regeneration and somatic embryogenesis in hypocotyl cultures. Proc. Indian natn. Sci. Acad. (Plant Sci.)93, 19–27.Google Scholar
  4. Binding, H., 1974: Regeneration von haploiden und diploiden Pflanzen aus Protoplasten vonPetunia hybrida L. Z. Pflanzenphysiol.74, 327–356.Google Scholar
  5. Button, J., Kochba, J., Bornman, C. H., 1974: Fine structure and embryoid development from embryogenic ovular callus of “Shamouti” orange (Citrus sinensis Osb.) J. exp. Bot.25, 446–457.Google Scholar
  6. Dodds, J. H., Roberts, L. W., 1982: Experiments in plant tissue culture. London: Cambridge Univ. Press.Google Scholar
  7. Gamborg, O. L., Miller, R. A., Ojima, K., 1968: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res.50, 151–158.Google Scholar
  8. Galun, E.,Aviv, D.,Raveh, D.,Vardi, A.,Zelcer, A., 1977: Protoplasts in studies of cell genetics and morphogenesis. In: Proc. Life Sci. (Reinhard, E.,Alfermann, A. W., eds.), pp. 301–312. Berlin.Google Scholar
  9. Haccius, B., 1978: Question of unicellular origin of non-zygotic embryos in callus cultures. Phytomorphology28, 74–81.Google Scholar
  10. Konar, R. N., Thomas, E., Street, H. E., 1972: Origin and structure of embryoids arising from epidermal cells of the stem ofRanunculus sceleratus L. J. Cell. Sci.11, 77–93.Google Scholar
  11. Lakshmi Sita, G., Raghava Ram, N. V., Vaidyanathan, C. S., 1979: Differentiation of embryoids and plantlets from shoot callus of sandalwood. Plant Sci. Lett.15, 265–270.Google Scholar
  12. — — —, 1980 a: Triploid plants from endosperm cultures of sandalwood by experimental embryogenesis. Plant Sci. Lett.20, 63–69.Google Scholar
  13. —,Shoba, G., Vaidyanathan, C. S., 1980 b: Regeneration of whole plants by embryogenesis from cell suspension cultures of sandalwood. Curr. Sci.49, 196–198.Google Scholar
  14. Murashige, T., Skoog, F., 1962: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.15, 473–497.Google Scholar
  15. Rangaswamy, N. S., Rao, P. S., 1963: Experimental studies onSantalum album L.—establishment of tissue culture of endosperm. Phytomorphology13, 450–454.Google Scholar
  16. Rao, P. S., 1965:In vitro induction of embryonal proliferation inSantalum album L. Phytomorphology15, 165–167.Google Scholar
  17. —, 1982: Protoplast culture. In: Experimental embryology of vascular plants (Johri, B. M., ed.), pp. 231–262. Berlin-Heidelberg-New York: Springer.Google Scholar
  18. —,Rangaswamy, N. S., 1971: Morphogenic studies in tissue cultures of the parasiteSantalum album L. Biol. Plant. (Praha)13, 200–206.Google Scholar
  19. —,Bapat, V. A., 1978: Vegetative propagation of sandalwood plants through tissue culture. Can. J. Bot.56, 1153–1156.Google Scholar
  20. — —, 1980: Morphogenetic investigations in tissue and organ cultures of sandalwood tree. In: Plant tissue culture, genetic manipulation and somatic hybridization of plant cells (Rao, P. S., Heble, M. R., Chadha, M. S., eds.), pp. 206–215. Proc. Natl. Symp., Bhabha Atomic Research Centre, Bombay.Google Scholar
  21. Rao, P. S., Raghava Ram, N. V., 1983: Propagation of sandalwood (Santalum album L.) using tissue and organ culture technique. In: Plant cell culture in crop improvement (Sen, S. K., Giles, K. L., eds.), pp. 419–422. New York: Plenum Publishing Corporation.Google Scholar
  22. —,Bapat, V. A., Mhatre, M., 1984: Regulatory factors forin vitro multiplication of sandalwood tree (Santalum album L.) II. Plant regeneration in nodal and internodal stem explants and occurence of somaclonal variations in tissue culture raised plants. Proc. Indian natn. Sci. Acad.B50, 148–154.Google Scholar
  23. Redenbaugh, K., Karnosky, D., Westfall, R. D., 1981: Protoplast isolation and fusion in threeUlmus species. Can. J. Bot.59, 1436–1443.Google Scholar
  24. Schieder, O., Vasil, I. K., 1980: Protoplast fusion and somatic hybridization. In: Perspectives in plant cell and tissue culture (Vasil, I. K., ed.), pp. 21–46. Int. Rev. Cytol. Suppl. 11 B. New York: Academic Press.Google Scholar
  25. Smith, M. A., McCown, B. H., 1982: A comparison of source tissue for protoplast isolation from three woody plant species. Plant Sci. Lett.28, 149–156.Google Scholar
  26. Tisserat, B., Esan, E. B., Murashige, T., 1979: Somatic embryogenesis in angiosperms. Hort. Rev.1, 1–78.Google Scholar
  27. Vardi, A., Spiegel-Roy, P., Galun, E., 1975:Citrus cell culture: isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos. Plant Sci. Lett.4, 231–236.Google Scholar
  28. — —, 1982: Plant regeneration fromCitrus protoplasts: variability in methodological requirements among cultivars and species. Theor. appl. Genet.62, 174–176.Google Scholar
  29. Vasil, I. K., Vasil, V., 1980: Isolation and culture of protoplasts. In: Perspectives in plant cell and tissue culture (Vasil, I. K., ed.), pp. 1–19. Int. Rev. Cytol. Suppl. 11 B. New York: Academic Press.Google Scholar
  30. Vasil, V., Vasil, I. K., 1982: Characterization of an embryogenic cell suspension culture derived from cultured inflorescences ofPennisetum americanum (pearl millet,Gramineae). Amer. J. Bot.69, 1441–1449.Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • P. S. Rao
    • 1
  • Peggy Ozias-Akins
    • 1
  1. 1.Independent Research Group SchiederMax-Planck-Institut für Züchtungsforschung (Erwin-Baur-Institut)Köln

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