, Volume 124, Issue 1–2, pp 80–86 | Cite as

Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood (Santalum album L.)

  • P. S. Rao
  • Peggy Ozias-Akins


Protoplasts were isolated from embryogenic cell suspension cultures derived from proliferating shoot segments of a 20-year-old sandalwood tree (Santalum albumLinn.). Under appropriate conditions, isolated protoplasts divided in liquid culture medium and produced embryogenic cell aggregates and globular embryos. Plating of cell aggregates on a fresh medium facilitated the differentiation of somatic emryos which further developed into plantlets.


Santalum album L. Sandalwood Somatic embryogenesis tree protoplasts 





2,4-dichlorophenoxyacetic acid


Gibberellic acid


indoleacetic acid


indolebutrytic acid


2-(N-morpholino)ethane sulfonic acid


Murashige and Skoog's medium as modified in the text


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • P. S. Rao
    • 1
  • Peggy Ozias-Akins
    • 1
  1. 1.Independent Research Group SchiederMax-Planck-Institut für Züchtungsforschung (Erwin-Baur-Institut)Köln

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