Summary
The fine structure of the testicular interstitial cells of the 9-day-old mouse submitted to stimulation with human chorionic gonadotropin (HCG) is reported. As was previously described (Baillie 1964) the interstitial tissue of the prepubertal mouse testis is characterized by the presence of well differentiated epithelioid cells at a quiescent stage. They are characterized by large cytoplasmic depots of lipid droplets and glycogen particles in contrast to poorly developed membranous organelles. These cells are highly sensitive to the action of gonadotropins. Five daily injections of HCG cause their differentiation into cells with active secretory characteristics. The gonadotropin induces a marked depletion of the lipid droplets and glycogen content of the cytoplasm, concurrent with an unusual development of the membranes of the agranular endoplasmic reticulum. Golgi complexes and rough reticulum are prominent. Several changes also appear in the nucleus, especially in the nucleolus.
The correlation of the present electron microscopic study of the interstitial cells under HCG stimulation with previous biochemical and physiological findings tentatively suggests that the immature Leydig cells exhibit the basic organization necessary for biosynthesis of steroid hormones.
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References
Albert, A., 1961: The mammalian testis. In: Sex and Internal Secretion (W. C. Young, ed.) Pp. 305–365. Baltimore: Williams and Wilkins Co.
Aldman, B., L. Claesson, N.-Å. Hillarp, andE. Odeblad, 1949: Studies on the storage mechanism of the oestrogen-precursor. Acta endocr.2, 24–32.
Aoki, A., 1968: Hormone-induced differentiation of agranular endoplasmic reticulum in the interstitial cells of the mouse testis. Protoplasma66, 263–267.
—, andL. S. Gutierrez, 1967: A simple toluidine blue-basic fuchsin stain for spermatozoa in epoxy sections. Stain Technol.42, 307–310.
Baillie, A. H., 1964: Further observations on the growth and histochemistry of the Leydig tissue in the postnatal prepubertal mouse testis. J. Anat.98, 403–419.
Bennett, H. S., andJ. H. Luft, 1959: s-Collidine as a basis for buffering fixatives. J. biophys. biochem. Cytol.6, 113–114.
Bishop, D. H., andJ. H. Leathem, 1946: Response of prepuberal male mice to equine gonadotropin. Anat. Rec.95, 313–319.
Black, V. H., andA. K. Christensen, 1969: Differentiation of interstitial cells and Sertoli cells in fetal guinea pig testes. Amer. J. Anat.124, 211–219.
Brinck-Johnsen, T., andK. B. Eik-Nes, 1957: Effect of human chorionic gonadotropin on the secretion of testosterone and 4-androstene-3, 17-dione by the canine testis. Endocrinology61, 676–683.
Carr, I., andJ. Carr, 1962: Membranous whorls in the testicular interstitial cells. Anat. Rec.144, 143–147.
Christensen, A. K., 1965: The fine structure of testicular interstitial cells in guinea pigs. J. Cell Biol.26, 911–935.
—, andD. W. Fawcett, 1966: The fine structure of testicular interstitial cells in mice. Amer. J. Anat.118, 551–572.
Claesson, L., 1954: The intracellular localization of the estenfied cholesterol in the living interstitial gland cell of the rabbit ovary. Acta physiol. scand.31 (Suppl. 113), 53–78.
Connel, G. M., C. J. Connel, andK. Eik-Nes, 1966: Testosterone synthesis by the two-day-old chick testisin vitro. Gen. comp. Endocr.7, 158–165.
Dallner, G., P. Siekevitz, andG. E. Palade, 1966: Biogenesis of endoplasmic reticulum membranes. I. Structural and chemical differentiation in developing rat hepatocyte. J. Cell Biol.30, 73–96.
De Kretzer, D. M., 1967: Changes in the fine structure of the human testicular interstitial cells after treatment with human gonadotrophins. Z. Zellforsch.83, 344–358.
—, 1967: The fine structure of the testicular interstitial cells in men of normal androgenic status. Z. Zellforsch.80, 594–609.
Diczfalusy, E., H. J. Holmgren, andA. Westman, 1950: The action of human chorionic gonadotrophin preparations on the adrenals and testicles of hypophysectomized immature rats. Acta endocr.5, 43–53.
Eik-Nes, K. B., 1964: Effects of gonadotrophins on secretion of steroids by the testis and ovary. Physiol. Rev.44, 609–630.
Fawcett, D. W., 1965: Structural and functional variations in the membranes of the cytoplasm. In: Intracellular membranous structure (S.Seno and E. V.Cowdry, eds.). Pp. 15–40. Japan Society for Cell Biology.
Greep, R. O., H. L. Fevold, andF. L. Hisaw, 1936: Effects of two hypophyseal gonadotropic hormones on the reproductive system of the male rat. Anat. Rec.65, 261–271.
Hall, P. F., 1966: On the stimulation of testicular steroidogenesis in the rabbit by interstitial cell-stimulating hormone. Endocrinology78, 690–698.
—, andK. B. Eik-Nes, 1962: The action of gonadotropic hormones upon rabbit testisin vitro. Biochim. Biophys. Acta63, 411–422.
— —, 1963: The influence of gonadotrophinsin vivo upon the biosynthesis of androgens by homogenate of rat testis. Biochim. Biophys. Acta71, 438–447.
—,E. E. Nishizawa, andK. B. Eik-Nes, 1963: Biosynthesis of testosterone by rabbit testis: homogenate V slices. Proc. Soc. exp. Biol. (N.Y.)114, 791–794.
Hitzeman, J. W., 1962: Development of enzyme activity in the Leydig cells of the mouse testis. Anat. Rec.143, 351–362.
Hooker, C. W., 1944: The postnatal history and function of the interstitial cells of the testis of the bull. Amer. J. Anat.74, 1–37.
—, 1948: Biology of interstitial cells of testis. Recent Progr. Hormone Res.3, 173–195.
Karnovsky, M. J., 1965: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol.27, 137 A.
Lindner, H. R., 1961: Androgens and related compounds in the spermatic vein blood of domestic animals. I. Neutral steroids secreted by the bull testis. J. Endocr.23, 139–159.
Long, J. A., andA. L. Jones, 1967: The fine structure of the zona glomerulosa and the zona fasciculata of the adrenal cortex of the opossum. Amer. J. Anat.120, 463–488.
Mancini, R. E., O. Vilar, J. C. Lavieri, J. A. Andrada, andJ. J. Heinrich, 1963: Development of Leydig cells in the normal human testis. A cytological, cytochemical, and quantitative study. Amer. J. Anat.112, 203–214.
Mason, N. R., andL. T. Samuels, 1961: Incorporation of acetate-1-C14 into testosterone and 3β-hydroxysterols by the canine testis. Endocrinology68, 899–907.
Menon, K. M. J., R. I. Dorfman, andE. Forchielli, 1967: Influence of gonadotrophins on the cholesterol-sidechain cleavage reaction by rat-testis mitochondrial preparations. Biochim. Biophys. Acta148, 486–494.
Mollenhauer, H. H., 1964: Plastic embedding mixtures for use in electron microscopy. Stain Technol.39, 111–114.
Moore, C. R., 1936: Responses of immature rat testes to gonadotropic agents. Amer. J. Anat.59, 63–88.
Moses, H. L., W. W. Davis, A. S. Rosenthal, andL. D. Garren, 1969: Adrenal cholesterol: Localization by electron-microscope autoradiography. Science163, 1203–1205.
Murota, S., M. Shikita, andB. Tamaoki, 1965: Intracellular distribution of the enzymes related to androgen formation in mouse testes. Steroids5, 409–413.
Pollock, W. F., 1942: Histochemical studies of the interstitial cells of the testis. Anat. Rec.84, 865–869.
Roosen-Runge, E. C., andD. Anderson, 1959: The development of the interstitial cells in the testis of the albino rat. Acta Anat.37, 125–137.
Saez, J. M., andJ. Bertrand, 1968: Studies on testicular function in children: Plasma concentration of testosterone, dehydroepiandrosterone, and its sulfate before and after stimulation with human chorionic gonadotrophin. Steroids12, 749–761.
Sayers, G., M. A. Sayers, E. G. Fry, A. White, andC. N. H. Long, 1944: The effect of the adrenotrophic hormone of the anterior pituitary on the cholesterol content of the adrenals. With a review of the literature on adrenal cholesterol. Yale J. Biol. Med.16, 361–392.
Schalch, D. S., A. F. Parlow, R. C. Boom, andS. Reichlin, 1968: Measurement of human luteinizing hormone in plasma by radioimmunoassay. J. Clin. Invest.47, 665–678.
Schoen, E. J., andL. T. Samuels, 1965: Testicular androgen biosynthesis following corticotrophin and human chorionic gonadotrophin administration. Acta endocr.50, 365–378.
Schwarz, W., undH. J. Merker, 1965: Die Hodenzwischenzellen der Ratte nach Hypophysektomie und nach Behandlung mit Choriogonadotropin und Amphenon B. Z. Zellforsch.65, 272–284.
Shikita, M., andB. Tamaoki, 1965: Testosterone formation by subcellular particles of rat testes. Endocrinology76, 563–569.
Toren, D., K. M. J. Menon, E. Forchielli, andR. I. Dorfman, 1964:In vitro enzymatic cleavage of the cholesterol side chain in rat testis preparations. Steroids3, 381–390.
Venable, J. H., andR. Coggeshall, 1965: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol.25, 407–408.
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Aoki, A. Hormonal control of leydig cell differentiation. Protoplasma 71, 209–225 (1970). https://doi.org/10.1007/BF01279632
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DOI: https://doi.org/10.1007/BF01279632