Summary
Protoplasts isolated from tobacco leaves showed the development of crystalline bodies in the chloroplasts and osmiophilic droplets in the chloroplasts and cytoplasm. Microbodies stained for catalase with the diaminobenzidine (DAB) reaction although, in the absence of H2O2, mitochondrial cristae also showed a dense reaction. Similar changes and DAB staining were observed in intact leaf cells aged in media similar to that used for protoplast isolation. The cell wall and plasma membrane of fresh leaf cells stained inconsistently with silicotungstic acid (STA). In comparison, the plasma membrane of freshly isolated protoplasts stained very poorly with STA although more intense staining was found in aged protoplasts. The surface of protoplasts showed heavy staining when incubated in lanthanum nitrate or cationized ferritin though not with ruthenium red. The potential of these surface stains as plasma membrane markers in isolated membrane fractions is discussed.
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Taylor, A.R.D., Hall, J.L. Fine structure and cytochemical properties of tobacco leaf protoplasts and comparison with the source tissue. Protoplasma 96, 113–126 (1978). https://doi.org/10.1007/BF01279580
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DOI: https://doi.org/10.1007/BF01279580