Skip to main content
Log in

Phagocytoseverhalten vonPseudomicrothorax dubius unter Normalbedingungen und nach Applikation von Colchicin sowie Cytochalasin B

Phagocytic behaviour ofPseudomicrothorax dubius under normal conditions and after application of colchicine and cytochalasin B

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

The phagocytic behaviour ofPseudomicrothorax dubius-a ciliate with a cytopharyngeal basket-was investigated under normal conditions as well as after application of colchicine and cytochalasin B. Colchicine, applied in concentrations of 0.03–0.05%, does not show a significant influence upon normal occuring food uptake. Higher concentrations cause finally cell's death. On the other hand, cytochalasin B blocks the phagocytosis entirely when applicated at concentrations of 7 μg/ml and more; membrane limited bubbles appear at the buccal opening. The findings are discussed with regard to the mechanisms and the generating forces of the process of food ingestion.

Zusammenfassung

Das Phagocytoseverhalten des mit einem Reusenapparat ausgestatteten CiliatenPseudomicrothorax dubius wurde unter Normalbedingungen sowie nach Applikation von Colchicin und Cytochalasin B untersucht. Bei Konzentrationen von 0,03–0,05% Colchicin — höhere Konzentrationen zeigten letale Wirkungen auf die Organismen — konnte kein signifikanter Einfluß auf die Nahrungsaufnahme festgestellt werden. Andererseits blockierte Cytochalasin B ab Konzentrationen von 7 μg/ml die Phagocytose vollständig; membranumgebene Blasen erschienen an der Buccalöffnung. Die Ergebnisse werden hinsichtlich der Mechanismen und der Krafterzeugung für die Nahrungsaufnahme diskutiert.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Literatur

  • Allison, A. C., andP. Davies, 1974: Mechanisms of endocytosis and exocytosis. Soc. Exp. Biol.28, 419–446.

    Google Scholar 

  • Bardele, C. F., 1972: A microtubule model for ingestion and transport in the suctorian tentacle. Z. Zeilforsch.126, 116–134.

    Google Scholar 

  • —, 1973: Struktur, Biochemie und Funktion der Mikrotubuli. Cytobiologie7, 442–488.

    Google Scholar 

  • —, 1974: Transport of materials in the suctorian tentacle. Soc. Exp. Biol.28, 191–208.

    Google Scholar 

  • Chapman-Andresen, C., 1962: Studies on pinocytosis inAmoebae. C. R. Lab. Carlsberg33, 73–364.

    Google Scholar 

  • —, 1973: Endocytic processes. In: The biology ofAmoeba (K. W. Jeon, ed.), pp. 319–348. New York-London: Academic Press.

    Google Scholar 

  • —, andM. Müller, 1974: Mechanisms of ingestion and digestion, secretion-excretion. In: Actualités protozoologiques (P. de Puytorac etJ. Grain, eds.), pp. 265–277. Clermont-Ferrand: Paul Couty.

    Google Scholar 

  • Corliss, J. O., 1958: The systematic position ofPseudomicrothorax dubius, a ciliate with a unique combination of anatomical features. J. Protozool.5, 184–193.

    Google Scholar 

  • Fauré-Fremiet, E., etJ. André, 1967: Etude au microscope électronique du ciliéPseudomicrothorax dubius Maupas. J. Protozool.14, 464–473.

    PubMed  Google Scholar 

  • Hausmann, K., 1975: Monströses Wachstum beiPseudomicrothorax dubius als Folge der Wirkung von Plastik auf die Nähralgen (Oscillatoria formosa). Protoplasma85, 1–14.

    PubMed  Google Scholar 

  • Hepler, P. K., andB. A. Palevitz, 1974: Microtubules and microfilaments. Ann. Rev. Plant Physiol.25, 309–362.

    Google Scholar 

  • Hoffmann, E. K., L. Rasmussen, andE. Zeuthen, 1974: Cytochalasin B: Aspects of phagocytosis in nutrient uptake inTetrahymena pyriformis. J. Cell Sci.15, 403–406.

    PubMed  Google Scholar 

  • Hull, R. W., 1961: Studies on suctorian protozoa: The mechanism of ingestion of prey cytoplasm. J. Protozool.8, 351–359.

    Google Scholar 

  • Nilsson, J. R., 1976: Physiological and structural studies onTetrahymena pyriformis GL. C. R. Lab. Carlsberg40, 215–355.

    Google Scholar 

  • —,T. R. Ricketts, andE. Zeuthen, 1973: Effects of cytochalasin B on cell division and vacuole formation inTetrahymena pyriformis GL. Exp. Cell Res.79, 456–459.

    PubMed  Google Scholar 

  • Peck, R. K., 1971: Fine structure, morphogenesis and interrelationships within representatives of three ciliated protozoan genera. Ph. D. Thesis, University of Illinois, Urbana.

    Google Scholar 

  • Pyne, C. K., etM. Tuffrau, 1970: Structure et ultrastructure de l'appareil cytopharyngien et des tubules complexes en relation avec celui-ci chez le cilié gymnostomeChilodonella uncinata Ehrgb. J. Microscopie9, 503–516.

    Google Scholar 

  • Stockem, W., 1973: Endocytose und intrazelluläre Verdauung. Bio. i. u. Zeit3, 67–78.

    Google Scholar 

  • —, andK. E. Wohlfarth-Bottermann, 1969: Pinocytosis (endocytosis). In: Handbook of molecular cytology (A. Lima de Faria, ed.), pp. 1373–1400. Amsterdam: North Holland Publishing Company.

    Google Scholar 

  • Thompson, J. C., andJ. O. Corliss, 1958: A redescription of the holotrichous ciliatePseudomicrothorax dubius with particular attention to its morphogenesis. J. Protozool.5, 175–184.

    Google Scholar 

  • Tucker, J. B., 1968: Fine structure and function of the cytopharyngeal basket in the ciliateNassula. J. Cell Sci.3, 493–514.

    PubMed  Google Scholar 

  • —, 1972: Microtubule-arms and propulsion of food particles inside a large feeding organelle in the ciliatePhascolodon vorticella. J. Cell Sci.10, 883–903.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hausmann, K., Eisenbarth, A. Phagocytoseverhalten vonPseudomicrothorax dubius unter Normalbedingungen und nach Applikation von Colchicin sowie Cytochalasin B. Protoplasma 92, 269–279 (1977). https://doi.org/10.1007/BF01279464

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01279464

Navigation