Summary
The developing primary leaves of mung bean seedlings contain plastids, called proteoplasts, which are modified for protein storage. The proteoplast has a large protein inclusion with a granular matrix that is bound by a single membrane. Proteoplasts of this type are located in a layer of tissue one cell thick between the palisade and spongy mesophyll cells. The cell layer containing proteoplasts (P layer) differentiates within a few days after seed imbibition. Proteoplast precursors are distinguished by the development of membrane-bound protein sacs within the stroma. The protein sacs coalesce to form a spherical protein body. The P layer is short lived in primary leaves of seedlings grown in light and degeneration of these cells begins soon after proteoplast differentiation. As the cell layer degenerates, proteoplast contents become very electron dense. Within two days, the P layer breaks down and disappears as adjacent cells enlarge and differentiate. In contrast, this specialized cell layer remains intact, with little change in proteoplast fine structure, over a corresponding period in etiolated seedlings.
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References
Ames, I. H., andJ. P. Pivorun, 1974: A cytochemical investigation of a chloroplast inclusion. Amer. J. Bot.61, 794–797.
Avery, G. S., 1933: Structure and development of the tobacco leaf. Amer. J. Bot.20, 565–595.
Bain, J. M., 1967: A crystalline inclusion in the chloroplasts of the outer hypodermal cells of the banana fruit. Aust. J. biol. Sci.21, 421–427.
Blackwell, S. J., W. M. Laetsch, andB. B. Hyde, 1969: Development of chloroplast fine structure in aspen tissue culture. Amer. J. Bot.56, 457–463.
Butler, W. L., J. De Greef, T. F. Roth, andH. Oelze-Karow, 1972: The influence of carbonylcyanide-m-chlorophenolhydrazone and 3-(3,4-dichlorophenyl)-1,1-dimethylurea on the fusion of primary thylakoids and the formation of crystalline fibrils in bean leaves partially greened in far red light. Plant Physiol.49, 102–104.
Cran, D. G., andJ. V. Possingham, 1972: Variation of plastid types in spinach. Protoplasma74, 345–356.
— —, 1974: Plastid thylakoid formation. Ann. Bot.38, 843–847.
De Greef, J. A., andJ. P. Verbelen, 1973: Physiological stress and crystallites in leaf plastids ofPhaseolus vulgaris L. Ann. Bot.37, 593–596.
Feder, N., andT. B. O'Brien, 1968: Plant microtechnique: Some principles and new methods. Amer. J. Bot.55, 123–142.
Foster, A. S., 1936: Leaf differentiation in angiosperms. Bot. Rev.2, 349–372.
Grilli, M. C., 1970: Crystalline inclusions in cycads root nodules. Giron. Bot. Ital.104, 75–79.
Heltne, J., andH. T. Bonnett, 1970: Chloroplast development in isolated roots ofConvolvulus arvensis (L.). Planta92, 1–12.
Hoefert, L. L., andK. Esau, 1975: Plastid inclusions in epidermal cells ofBeta. Amer. J. Bot.62, 36–40.
Israel, H. W., andF. C. Steward, 1967: The fine structure and development of plastids in cultured cells ofDaucus carota. Ann. Bot.31, 1–18.
Jensen, T. E., andJ. G. Valdovinos, 1967: Fine structure of abscission zones. I. Abscission zones of the pedicels of tobacco and tomato flowers at anthesis. Planta77, 298–318.
Klein, S., andB. M. Pollock, 1968: Cell fine structure of developing lima bean seeds related to seed desication. Amer. J. Bot.55, 658–672.
Lee, R. E., andA. Thompson, 1973: The stromacentre of plastids ofKalanchoË pinatta Persoon. J. Ultrastruct. Res.42, 451–456.
Maksymowych, R., andR. O. Erickson, 1960: Development of the lamina inXanthium italicum represented by the plastochrom index. Amer. J. Bot.47, 451–459.
Marinos, N. G., 1967: Multifunctional plastids in the meristematic region of potato buds. J. Ultrastruct. Res.17, 91–113.
Mazia, D., P. A. Brewer, andM. Alfert, 1953: The cytochemical staining and measurement of protein with mercuric bromphenol blue. Biol. Bull.104, 57–67.
Mills, G. L., andE. C. Cantino, 1975: The single microbody in the zoospore ofBlastocladiella emersonii is a “symphyomicrobody”. Cell Differentiation4, 35–43.
Mohr, W. P., andM. Stein, 1969: Fine Structure of fruit development in tomato. Can. J. Plant Sci.49, 549–553.
Mollenhauer, H. H., 1964: Plastic embedding mixtures for use in electron microscopy. Stain Tech.39, 111–114.
Mühlethaler, K., 1971: The ultrastructure of plastids. In: Structure and function of chloroplasts (M. Gibbs, ed.), pp. 7–34. New York: Springer-Verlag.
Newcomb, E. H., 1967: Fine structure of protein-storing plastids in bean root tips. J. Cell Biol.33, 143–163.
Oliveira, L., 1975: On the morphology and nature of the plastid inclusions of leaf cells of a Triticale. J. submicr. Cytol.7, 271–280.
Philipson, W. R., 1949: The ontogeny of the shoot apex in dicotyledons. Biol. Rev.24, 21–50.
Price, J. L., andW. W. Thomson, 1967: Occurrence of a crystalline inclusion in the chloroplasts ofMacadamia leaves. Nature214, 1148–1149.
Rosinski, J., andW. G. Rosen, 1972: Chloroplast development: Fine structure and chlorophyll synthesis. Quart. Rev. Biol.47, 160–191.
Salema, R., J. F. Mesquita, andI. Abreu, 1972: Particular aspects of the construction of photosynthetic membranes. J. submicr. Cytol.4, 161–169.
Sass, J. E., 1958: Botanical microtechnique. Ames, Iowa: The Iowa State University Press.
Schiff, J. A., 1972: A green safelight for the study of chloroplast development and other photomorphogenetic phenomena. Meth. Enz.24, 321.
Shumway, L. K., T. E. Weier, andC. R. Stocking, 1967: Crystalline structures inVicia faba chloroplasts. Planta76, 182–189.
Shushan, S., andM. A. Johnson, 1955: The shoot apex ofDianthus caryophyllus L. Bull. Torrey Bot. Club82, 266–283.
Srivastava, L. M., 1966: On the fine structure of the cambium ofFraxinus americana L. J. Cell Biol.31, 79–93.
Stetler, D. A., andW. M. Laetsch, 1969: Chloroplast development inNicotiana tabacum “Maryland Mammoth”. Amer. J. Bot.56, 260–270.
Tulett, A. J., V. Bagshaw, andM. M. Yeoman, 1969: Arrangement and structure of plastids in dormant and cultured tissue from artichoke tubers. Ann. Bot.33, 217–226.
Whatley, J. M., 1974: Chloroplast development in primary leaves ofPhaseolus vulgaris. New Phytol.73, 141–150.
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Hurkman, W.J., Kennedy, G.S. Fine structure and development of proteoplasts in primary leaves of mung bean. Protoplasma 89, 171–184 (1976). https://doi.org/10.1007/BF01279337
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DOI: https://doi.org/10.1007/BF01279337